共振能量转移技术在单采血小板保存过程功能监测中的应用研究*

doi:10.3969/j.issn.1671-2587.2026.04.025

临床输血与检验 ›› 2026, Vol. 28 ›› Issue (4): 628-636.DOI: 10.3969/j.issn.1671-2587.2026.04.025

共振能量转移技术在单采血小板保存过程功能监测中的应用研究^*

王涛¹, 王晓清¹, 马巧琴¹, 何林燕²

¹常州市中心血站,江苏常州 213012;
²苏州大学附属第一医院,江苏省血液研究所,国家血液系统疾病临床医学研究中心,国家卫生健康委员会血栓与止血重点实验室,血液学协同创新中心,江苏苏州 215006

收稿日期:2026-07-06 出版日期:2026-08-20 发布日期:2026-08-26
通讯作者: 何林燕,主要从事血小板血栓与止血的基础和临床研究,（E-mail）linyanhe2021@126.com。
作者简介:王涛,主要从事输血技术方面研究,（E-mail）wangtaaao@126.com。
基金资助:
^*本课题受常州市卫健委科技项目（指导性项目）（No.WZ202506）资助

Application of Chemiluminescence Resonance Energy Transfer Technology in Functional Monitoring of Apheresis Platelets during Storage

WANG Tao¹, WANG Xiaoqing¹, MA Qiaoqin¹, HE Linyan²

¹Changzhou Central Blood Station; Changzhou, Jiangsu, 213012;
²Jiangsu Institute of Hematology, NHC Key Laboratory of Thrombosis and Hemostasis, National Clinical Research Center for Hematologic Diseases, Collaborative Innovation Center of Hematology, Suzhou, Jiangsu 215006

Received:2026-07-06 Online:2026-08-20 Published:2026-08-26

摘要/Abstract

摘要： 目的探讨共振能量转移技术在单采血小板保存过程功能监测中的应用价值,结合流式细胞术检测评价其用于血小板膜分子及活化相关指标检测的可行性。方法随机收集2026年48例捐献者的单采血小板,随机保存1~7天,共7组。采用流式细胞术检测各组CD61表达率、CD61平均荧光强度、CD62P表达率和CD62P平均荧光强度;采用共振能量转移技术检测各组CD61、基础状态CD62P及ADP刺激后CD62P相关指标,并记录阳性表达率和相对化学发光强度。对共振能量转移技术进行批内精密度验证。结果流式CD62P表达率和CD62P平均荧光强度均随保存时间延长升高（P<0.05）。基础状态CRET-CD62P表达率与保存天数呈显著正相关（P<0.05）,基础状态CRET-CD62P发光数值与保存天数亦呈显著正相关（P<0.05）。ADP刺激后CRET-CD62P表达率和发光数值均较基础状态显著升高（P<0.05）,但以差值表示的反应储备与保存天数未见显著相关（P>0.05）。CRET-CD61发光数值与流式CD61平均荧光强度呈显著正相关（P<0.05）,但表达率相关性不足。重复性检测显示CD61低值、高值CV分别为6.25%、3.91%,CD62P低值、高值CV分别为14.27%、11.43%。结论共振能量转移技术可反映单采血小板保存过程中基础CD62P活化信号变化,并可检测ADP诱导活化反应,具有用于血小板保存质量快速监测的潜在价值。

关键词: 单采血小板, 共振能量转移技术, CD62P, CD61, 血小板活化, 流式细胞术

Abstract: Objective To investigate the application value of chemiluminescence resonance energy transfer (CRET) technology in functional monitoring of apheresis platelets during storage, and to evaluate its feasibility for detecting platelet membrane molecules and activation-related indicators in combination with flow cytometry. Methods Apheresis platelet samples were randomly collected from 48 donors in 2026 and randomly assigned to seven storage groups according to storage duration from day 1 to day 7. Flow cytometry was used to detect CD61 expression percentage, CD61 mean fluorescence intensity (MFI), CD62P expression percentage and CD62P MFI in each group. CRET technology was used to detect CD61, basal CD62P and ADP-stimulated CD62P-related indicators, and positive expression percentage and relative light units (RLU) were recorded. Intra-assay precision of the CRET method was evaluated. Results Flow cytometric CD62P expression percentage and CD62P MFI increased with prolonged storage time (P<0.05). Basal CRET-CD62P expression percentage was significantly positively correlated with storage duration (P<0.05), and basal CRET-CD62P RLU was also significantly positively correlated with storage duration (P<0.05). After ADP stimulation, both CRET-CD62P expression percentage and RLU were significantly higher than those in the basal state (P<0.05), whereas the response reserve expressed as the difference between ADP-stimulated and basal values showed no significant correlation with storage duration (P>0.05). CRET-CD61 RLU was significantly positively correlated with flow cytometric CD61 MFI (P<0.05), while the correlation between CD61 expression percentages detected by the two methods was insufficient. Repeatability testing showed that the coefficients of variation (CVs) of low- and high-level CD61 samples were 6.25% and 3.91%, respectively, and the CVs of low- and high-level CD62P samples were 14.27% and 11.43%, respectively. Conclusion CRET technology can reflect changes in basal CD62P-related activation signals during apheresis platelet storage and can detect ADP-induced platelet activation responses. It has potential value for rapid monitoring of platelet storage quality.

Key words: Apheresis platelet, Resonance energy transfer, CD62P, CD61, Platelet activation, Flow cytometry

中图分类号:

R457

王涛, 王晓清, 马巧琴, 何林燕. 共振能量转移技术在单采血小板保存过程功能监测中的应用研究^*[J]. 临床输血与检验, 2026, 28(4): 628-636.

WANG Tao, WANG Xiaoqing, MA Qiaoqin, HE Linyan. Application of Chemiluminescence Resonance Energy Transfer Technology in Functional Monitoring of Apheresis Platelets during Storage[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(4): 628-636.

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共振能量转移技术在单采血小板保存过程功能监测中的应用研究^*

Application of Chemiluminescence Resonance Energy Transfer Technology in Functional Monitoring of Apheresis Platelets during Storage

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