• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (2): 146-149.DOI: 10.3969/j.issn.1671-2587.2016.02.018

• 实验技术与方法 • 上一篇    下一篇

•实验技术与方法•冷沉淀不同融解方式对凝血因子活性的影响*

张婵,陈婉璐,程瑜静,李琦,代润,李清   

  1. 650032 云南省第一人民医院输血科
  • 收稿日期:2015-11-02 出版日期:2016-04-20 发布日期:2016-09-21
  • 作者简介:张婵(1969-),女,副主任技师,在读硕士研究生,主要从事血液免疫学、临床输血安全研究,（Tel）13619697064。 共同第一陈婉璐（1982-）,女,主治医师,学士,主要从事输血检测工作,（Tel）13608803742。
  • 基金资助:
    *本课题受云南省教育厅理工类重点项目（No.2014Z038)资助

Effects of Different Ways Melting Cryoprecipitate to Coagulation Factor Activity

ZHANG Chan, CHEN Wan-lu, CHENG Yu-jing, et al   

  1. The first people’s Hospital of Yunnan Province Kunming Yunnan 650032
  • Received:2015-11-02 Online:2016-04-20 Published:2016-09-21

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摘要： 目的探讨使用干式融浆机和水溶式融浆机融解冷沉淀及融解时间的长短对冷沉淀中凝血因子生物活性的影响,以指导输血科更好地保护冷沉淀中凝血因子的活性和选择正确的融浆仪器及最佳的融浆时间,为达到最好的融解效果提供理论依据。方法选取云南昆明血液中心提供的140袋冷沉淀（规格1.0 U/袋）,采集时间相近(不超过5 d),选取其中80袋冷沉淀作为1号检测组,1号检测组使用水浴式融浆机融解;剩下的60袋冷沉淀作为2号检测组,2号检测组选用干式融浆机融解。采用CA-1500全自动血凝分析仪,对140份置于不同融浆机37℃融解10、20、30 min的冷沉淀样品,分别测定FⅧ、VWF抗原、PT、TT、APTT及Fib。结果干式、水浴式两种仪器的融浆效果及各个检测指标相比,差异无统计学意义(P>0.05)。但随着融浆时间的延长,会导致APTT延长、FⅧ、VWF抗原活性下降,差异有统计学意义（P<0.05）。结论融浆时间延长会导致凝血因子FⅧ、VWF抗原活性下降,APTT延长,差异有统计学意义。为保障临床输血安全有效,推荐融解冷沉淀的最佳时间为10 min。

关键词: 不同融浆方式, 冷沉淀, 凝血因子, 融浆时间

Abstract: ObjectiveTo explore the effects on biological activity of coagulation factor in cryoprecipitate by using dry plasma and waterbath plasma melting instrument to melt cryoprecipitate and the length of melting time. Guiding transfusion department to protect the activity of coagulation factor better in cryoprecipitate and choose the right melting instrument as well as the opportune time. Providing a theoretical basis to achieve the best effect of melt. MethodChoosing 140 bags cryoprecipitate (specifications 1.0 U / bag) provided by Kunming Blood Center of Yunnan and the acquisition time is similar (not more than five days). Choosing 80 bags cryoprecipitate as test group 1 and using water-soluble plasma melting instrument to melt. Choosing the remaining 60 bags cryoprecipitate as test group 2 and using dry plasma melting instrument to melt. Using CA-1500 automatic coagulation analyzer to test FⅧ, VWF antigen, PT, TT, APTT and Fib of 140 copies cryoprecipitate samples of which are melted 10 minutes, 20 minutes, 30 minutes in different plasma melting instruments of 37 ℃. ResultThe effects of dry and water bath plasma melting instruments and various detection index compared with no significant difference, P> 0.05. But with the extension of melting time will lead to the APTT, FⅧ, VWF antigen activity decreased, P<0.05, the difference is significant. ConclusionProlonged the time of melting plasma will cause a decrease in coagulation factor FⅧ , VWF antigen activity and the effectiveness of APTT prolonged. There is a significant difference. In order to ensure the clinical blood transfusion is safe and effective, we recommend the best melting time of cryoprecipitate as 10 minutes.

Key words: Different, melting, method, Cold, precipitation, Clotting, factor, Melting, time

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