评估甘露醇-腺嘌呤-磷酸盐（MAP）溶液重悬洗涤红细胞的质量和储存期限*

doi:10.3969/j.issn.1671-2587.2025.03.016

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (3): 378-383.DOI: 10.3969/j.issn.1671-2587.2025.03.016

评估甘露醇-腺嘌呤-磷酸盐（MAP）溶液重悬洗涤红细胞的质量和储存期限^*

赵薇¹, 郑子薇², 卢发强¹, 杨宜承²

¹大连大学附属中山医院, 辽宁大连, 116001;
²大连市血液中心, 辽宁大连, 116001

收稿日期:2024-12-30 发布日期:2025-06-23
通讯作者: 卢发强,主要从事临床输血等方面的研究,（E-mail）faqiang3@163.com。共同通信作者杨宜承,主要从事临床输血、输血免疫等方面的研究,（E-mail）277745091@qq.com。
作者简介:赵薇,主要从事临床输血等方面的研究,（E-mail）39259239@qq.com。
基金资助:
*本课题受大连市医学科学研究计划项目（No.2011037）资助

Evaluating the Quality and Storage Duration of Washed Red Blood Cells Resuspended in Mannitol-adenine-phosphate (MAP) Solution

ZHAO Wei¹, ZHENG Ziwei², LU Faqiang¹, YANG Yicheng²

¹Affiliated Zhongshan Hospital of Dalian University;
²Dalian Blood Center 116001

Received:2024-12-30 Published:2025-06-23

摘要/Abstract

摘要： 目的比较MAP重悬洗涤红细胞与悬浮红细胞在储存过程中的细胞膜蛋白及pH的动态变化,确定MAP重悬洗涤红细胞的最适合保存期。方法将同一来源压积红细胞均分为两组,分别制备成悬浮红细胞和MAP重悬洗涤红细胞;在储存第2、7、14、21、28和35天,检测两组红细胞膜表面蛋白（CD47、CD55、CD59、CD147、PS外翻率）及pH值。结果 MAP重悬洗涤红细胞各检测时间点pH值显著低于悬浮红细胞（P<0.05）。储存至35天,与悬浮红细胞相比,MAP组CD47,CD55和CD59表达量明显降低（P<0.01）,PS外翻率明显升高（P<0.01）,CD147表达量下降但无统计学意义。红细胞膜蛋白变化与pH值存在显著相关性（P<0.01）。结论 MAP重悬洗涤红细胞在短期（≤21 d）储存中质量不低于悬浮红细胞,建议MAP重悬洗涤红细胞的最适保存期为21 d,以确保输注安全性与有效性。

关键词: 洗涤红细胞, 储存损伤, 质量评估, 膜蛋白

Abstract: Objective To compare membrane proteins dynamics and pH changes between MAP-suspended washed RBCs and suspended RBCs to determine the optimal storage period for MAP-suspended washed RBCs. Methods Packed RBCs were equally divided into two groups: suspended RBCs and MAP-suspended washed RBCs. Membrane protein expression (CD47, CD55, CD59, CD147) and PS externalization rate, along with pH values, were measured on storage days 2, 7, 14, 21, 28, and 35. Results The pH values of MAP-suspended washed RBCs were significantly lower than those of suspended RBCs at all timepoints (P<0.05). By day 35, MAP-suspended washed RBCs showed significantly reduced CD47, CD55, and CD59 expression (P<0.01), elevated PS externalization (P<0.01), and a non-significant decline in CD147. A significant correlation was observed between membrane protein changes and pH values (P<0.01). Conclusion The quality of MAP-suspended washed RBCs not inferior to that of suspended RBCs during short-term storage (≤21 days). It is recommended that the optimal storage period for MAP resuspended washed RBCs be 21 days to ensure the safety and efficacy of transfusion.

Key words: Washed RBCs, Storage duration, Quality evaluation, Membrane protein

中图分类号:

R331.1⁺41R457

赵薇, 郑子薇, 卢发强, 杨宜承. 评估甘露醇-腺嘌呤-磷酸盐（MAP）溶液重悬洗涤红细胞的质量和储存期限^*[J]. 临床输血与检验, 2025, 27(3): 378-383.

ZHAO Wei, ZHENG Ziwei, LU Faqiang, YANG Yicheng. Evaluating the Quality and Storage Duration of Washed Red Blood Cells Resuspended in Mannitol-adenine-phosphate (MAP) Solution[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(3): 378-383.

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评估甘露醇-腺嘌呤-磷酸盐（MAP）溶液重悬洗涤红细胞的质量和储存期限^*

Evaluating the Quality and Storage Duration of Washed Red Blood Cells Resuspended in Mannitol-adenine-phosphate (MAP) Solution

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