Abstract: ObjectiveTo Analyze the correlation of HBsAg detection results by ELISA with different S/CO values and gold-labeling methodfor safe collection of blood and reduction of the waste of blood resources. Methods37 966 volunteer blood donors were subjected to detection in Handan area from Nov 2014 to Apr 2015. After primary screening of HBsAg,gold-labeling method was used for re-detection of the samples that showed positive HBsAg in the previous detection by ELISA. The results of ELISA test were compared with those of gold-labeling. ResultsFrom Nov 2014 to Jan 2015,HBsAg was found to be positive in 102 cases of volunteer blood donors,with a positive rate of 0.52％. The gold-labeling detection rate seemed to be various in companies （χ²=33.33,P<0.01;χ²=25.47,P<0.01）. The false positivity was noted when re-tested by gold-labeling in all of the apecimens below the S/CO value of 20,Hereafter,the HBsAg- positive rate showed a gradual reduction（χ²=14.58,P<0.05）. ConclusionIt is an efficient way for reduction of false positivity of HBsAg to improve the gold-labeling method and operation skills of technicians.

Key words: Volunteer, blood, donors, S/CO, value, HBsAg, Relevant, research