Abstract: Objective To analyze the change of the scatter plots of white blood cell and basophilic granulocyte count channels （WBC/BASO）and explore the resean for false elevation of basophils count. Methods The data of scatter diagram of false elevation in Baso counts were collected that had been verified by manual counting in blood smears of 46 impatients. Results Three factors contributed to the false increase of Baso count,including abnomal lymphocyte （Abn-L）,neutrophil toxic granules （TG）and improper blood collection. Among them,Abn-L interference dominates（56.5%）. All of the samples of false elevated Baso counts with typical differential luekocye counts and Abn-L or TG WBC/Baso scatter diagram presented abnormal lymphocyte count or TG by manual counting in periphera blood smears. Differential leukocyte counting （DC）showed that the abnormal increase was originated from Abn-L or TG. Conclusion The samples with false increase of Baso count should be comfirmed by instrumental typical scatter diagram to provide orientation of repeated detection and correct data for clinical use.

Key words: Basophil, Scatter diagram, Basophil count