Abstract: Objective To investigate the current status of Staphylococcus aureus and streptococcus pneumoniae infection in our hospital from 2012 to 2016 and their drug resistance in order to provide reference for clinical medication．Methods The retrospective analysis was performed on the separation condition and the drug resistance of Staphylococcus aureus and streptococcus pneumoniae from the bacterial culture among the inpatients of our hospital from 2012 to 2016. Results A total of 18 966 strains of pathogenic bacteria were isolated from all kinds of specimens,and 1 697 strains of Staphylococcus aureus were isolated. Among them,623 strains were methicillin resistant Staphylococcus aureus （MRSA）,accounting for 36.71%,Streptococcus pneumoniae 353,and the isolation rate was 1.86%.The main sources of the samples were sputum,secretion and blood;the departments with Staphylococcus aureus infection were mainly the ICU,neurosurgery,orthopedics and geriatric ward。The isolation rate of SA in 5 years and the antimicrobial resistance rate showed a continuous slow downward trend,but the detection rate of MRSA had no obvious change。The Results of SP showed that no vancomycin or linezolid resistant strains were found,The resistance rates of erythromycin,clindamycin,tetracycline and sulfamethoxazole were higher,There was a certain fluctuation of drug sensitivity in 2012-2016 year.Conclusion Our hospital SA hospital infection control is better,but the drug resistance of SP isolated from our hospital was not optimistic. clinicians should still pay more attention to the clinical rational use of antibiotics according to drug susceptibility test Results.

Key words: Staphylococcus aureus, Streptococcus pneumoniae, Drug resistance, Trend