Abstract: Objective To investigate the application of ultrasound-guided venipuncture in outdoor blood collection. Methods A total of 486 blood donors with unsuccessful venipuncture collection were in even or odd divided into control group and observation group. Conventional venipuncturing was used for the control while ultrasound-guided operation for the observation group. The number,success frequency of puncturing,feeling of pain and compliance of the donors to venipuncture were observed. Results The rate of venipuncturing,frequency of success for each puncture,satisfaction and the pain score in observation group were （1.00 ± 0.00）,100.0% （243/243）,100.0% （243/243）,and （1.34 ± 0.62）,respectively when compared to （2.12 ± 1.05）,5.76% （14/243）,4.94% （12/243）,and （6.73 ± 2.07） in control group. The all indicators were significantly superior in venous puncturing method to those in the routine method （t= 3.064,χ²=433.05,χ²= 440.26,t= 5.148,all P<0.01）. Conclusion Ultrasound-guided venipuncture may remarkably improve the efficiency of routine venipuncture and reduce the pain of blood donors,which is practical value in outdoor blood collection.

Key words: Ultrasound guidance, Venipuncture, Blood donation