Sensitivity of Klebsiella Pneumoniae to Antibiotics and Its Genetic Distribution of Plasmid Mediated Resistance to Quinilone

doi:10.3969/j.issn.1671-2587.2020.04.021

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2020, Vol. 22 ›› Issue (4): 425-429.DOI: 10.3969/j.issn.1671-2587.2020.04.021

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Sensitivity of Klebsiella Pneumoniae to Antibiotics and Its Genetic Distribution of Plasmid Mediated Resistance to Quinilone

ZHAI Jun-bin, CAO Xiao-li, CHENG Li, et al.

Department of Laboratory Medicine, The Drum Tower Hospital of Nanjing University Medical College, Nanjing 210008

Received:2019-02-27 Online:2020-08-20 Published:2020-08-12

Abstract

Abstract: Objective To analyze the drug resistance of Klebsiella pneumoniae and its distribution of plasmid mediated resistant genes. Methods A total of 115 K. pneumoniae isolates were collected and the drug susceptibility was determined by microdilution. PCR and DNA sequencing were used to detect the prevalence of extended ß-lactmase, AmpC enzyme, carbapenemase, 16S rRNA methylase encoding genes and the plasmid meidated quinolone resistance loci. The bacteria strains were divided into 2 groups according to the MIC of imipenem, the difference of the genetic distribution was compared. Results Over 30 percent of isolates showed non-sensitivity to antibiotics, and blaCTX-M-14 dominated in the blaESBL. The highest prevalence of oqxAB was noted, accounting for 60.9% and obviously higher in CSKP than in the CRKP. In comparison, the prevalence of blaCTX-M and blaTEM as well as rmtB was higher in CNSKP than that in CSKP（P<0.05）. Conclusion The rational use of antimicrobial agents and the implementation of infection control measures should be emphasized in order to inhibit the occurance of CNSKP by restricting the spread of resistant elements.

Key words: Klebsiella, pneumoniae, ESBL, PMQRs, AmpC, 16, s-RMTase

CLC Number:

R378.98⁺6

ZHAI Jun-bin, CAO Xiao-li, CHENG Li, et al.. Sensitivity of Klebsiella Pneumoniae to Antibiotics and Its Genetic Distribution of Plasmid Mediated Resistance to Quinilone[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(4): 425-429.

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Sensitivity of Klebsiella Pneumoniae to Antibiotics and Its Genetic Distribution of Plasmid Mediated Resistance to Quinilone

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