Abstract: Objective Study on the isoimmunization of RhD negative pregnant women and explore the influencing factors of producing anti-D antibody,and to monitor and prevent hemolytic disease of newborn in perinatal period. Methods ABO blood group and RhD antigen were detected by the standard serological methods,RhD antigen negative confirmation and antibody screening were tested by indirect antiglobulin test. Asia Del（RHD*01EL.01）was detected by PCR-SSP. Results Among 596 pregnant women,70 were confirmed to be RhD negative,of whom 6 produced the anti-D antibody. No anti-D antibody were observed in all first-time pregnant and Del type women．In multi-pregnant women,8.57% produce anti-D allo-immune response. Conclusion Anti-D isoimmunization is affected by many factors and the level of anti-D is regularly monitored during perinatal period of RhD negative pregnant women. Anti-D immunoglobulin treatment is recommended for those pregnant women who do not produce anti-D,and appropriate treatment shoud be adopted to treat or prevent the newborns of pregnant women with anti-D.

Key words: Hemolytic, disease, of, the, fetus, and, newborn, RhIG, RhD, isoimmunization, Anti-D, antibody