Platelet Rich Fibrin Releasate Improves Atopic Dermatitis in Rats: an Experimental Study

doi:10.3969/j.issn.1671-2587.2023.01.012

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2023, Vol. 25 ›› Issue (1): 69-74.DOI: 10.3969/j.issn.1671-2587.2023.01.012

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Platelet Rich Fibrin Releasate Improves Atopic Dermatitis in Rats: an Experimental Study

WANG Qiao-gai, BI Li-bo, LIU Su-rui

Department of Clinical Laboratory, No. 980 Hospital, Joint Logistics Support Force, Shijiazhuang 050082

Received:2022-09-16 Published:2023-02-20

Abstract

Abstract: Objective This study was aimed to investigate the effect of PRF-r on the 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) rat model and explore its possible underlying mechanisms. Methods SD rats (including 10 rats required for PRF-r preparation) were divided into four groups: untreated rats (control group), AD-induced rats (DNCB group), AD-induced rats treated with Mometasone furoate (Mometasone furoate group) and AD-induced rats treated with PRF-r (PRF-r group), with 8 in each group. Non-anticoagulant caudal vein blood of normal rats was extracted to prepare PRF-r. AD was induced in the back of rats by repeated topical administration of DNCB. PRF-r and mometasone furoate groups were treated for 10 consecutive days from the 7th day. Clinical skin scoring was performed on the AD site. AD tissues were stained with hematoxylin eosin (H&E) to observe the pathological changes. Levels of interleukin-4 (IL-4) and γ-Interferon (IFN-γ) were determined by enzyme-linked immunosorbent assay (ELISA) in the sera of rats and by immunohistochemistry at the AD site. Results PRF-r significantly improved the AD-like symptoms, and decreased the dermatitis score compared with the DNCB induced model group(PRF-r group:1.8±0.27, DNCB induced model group: 4.9±0.44). ELISA analysis showed that compared to the serum levels of IFN-γ (140.43±8.21) pg/mL and IL-4 (206.63±2.17) pg/mL in the control group, IFN-γ in the DNCB induced model group was significantly reduced (114.21± 6.67) pg/mL, while IL-4 was increased markedly (221.71±2.58) pg/mL. Meanwhile, Mometasone furoate cream and PRF-r increased the serum level of IFN-γ (127.83±7.59) pg/mL, (145.66±7.92) pg/mL and brought IL-4 back to the normal level (210.45±4.15) pg/mL, (212.26±3.71) pg/mL respectively. Further immunohistochemical testing showed the levels of IFN-γ and IL-4 in each group tended to be consistent with those in the sera. Conclusion PRF-r may inhibit DNCB induced AD in rats by regulating Th2 type inflammatory response and Th1/2 cytokine level.

Key words: Platelet rich fibrin-releasate, Atopic dermatitis, Cytokine, Immune

CLC Number:

R392

WANG Qiao-gai, BI Li-bo, LIU Su-rui. Platelet Rich Fibrin Releasate Improves Atopic Dermatitis in Rats: an Experimental Study[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(1): 69-74.

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Platelet Rich Fibrin Releasate Improves Atopic Dermatitis in Rats: an Experimental Study

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