Knockdown of Hsa_Circ_0020080 Promotes Proliferation, Migration and Invasion of Human Breast Cancer Cells by Regulating Lipid Metabolism

doi:10.3969/j.issn.1671-2587.2025.01.005

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2025, Vol. 27 ›› Issue (1): 34-47.DOI: 10.3969/j.issn.1671-2587.2025.01.005

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Knockdown of Hsa_Circ_0020080 Promotes Proliferation, Migration and Invasion of Human Breast Cancer Cells by Regulating Lipid Metabolism

WANG Lingxia, LI Xiao, XU Shiliang, WANG Bo, YANG Huan

The Second Affiliated Hospital of Soochow University,Suzhou, Jiangsu 215000

Received:2024-05-23 Published:2025-02-25

Abstract

Abstract: Objective The purpose of this study was to explore the effect of knockdown of Hsa_circ_0020080 on the proliferation, migration and invasion of human breast cancer cells by regulating lipid metabolism. Methods Quantitative real-time fluorescence PCR (qRT PCR) was used to validate the efficiency of Hsa_circ_0020080 knockdown and the expression levels of MDM2, VEGF, FASN, HMGCR, CPT1A, SREBP1. Western blot was used to detect the expression levels of MDM2 and VEGF proteins. Cell proliferation function was examined in vitro using colony formation and CCK8 assays; Transwell migration and matrigel invasion experiments were conducted to detect cell migration and invasion functions. Transplanted tumor model of nude mice was established in vivo. Immunohistochemistry was used to detect the protein expression of MDM2, VEGF, FASN, HMGCR, and SREBP1ere detected by. Oil red O staining and triglyceride assay kit were used to detect the expression levels of triglyceride. Functional rescue experiments were conducted by the effective inhibitor of fatty acid synthase FASN (C75). Results The Knockdown of Hsa_circ_0020080 in vitro could increase the colony formation, proliferation, migration and invasion ability of breast cancer cells, promote the expression of MDM2, VEGF, FASN, HMGCR, CPT1A, SREBP1 genes and MDM2, VEGF proteins, and increase the triglyceride content of breast cancer cells; The Knockdown of Hsa_circ_0020080 in vivo promotes the growth of xenograft tumors in nude mice, increases the expression of MDM2, VEGF, FASN, HMGCR, SREBP1 proteins, and triglyceride levels in xenograft tumors of nude mice. C75 can partially rescue proliferation, migration and invasion ability of breast cancer cells with the knockdown of Hsa_circ_0020080. Conclusion Knockdown of Hsa_circ_0020080 promotes the proliferation, invasion and migration of breast cancer cells through lipid metabolism, which plays an important biological role in the development of breast cancer.

Key words: Hsa_circ_0020080, Breast cancer, Proliferation, Migration, Invasion, Lipid metabolism

CLC Number:

R737.9

WANG Lingxia, LI Xiao, XU Shiliang, WANG Bo, YANG Huan. Knockdown of Hsa_Circ_0020080 Promotes Proliferation, Migration and Invasion of Human Breast Cancer Cells by Regulating Lipid Metabolism[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(1): 34-47.

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Knockdown of Hsa_Circ_0020080 Promotes Proliferation, Migration and Invasion of Human Breast Cancer Cells by Regulating Lipid Metabolism

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