Abstract: Objective To investigate the genetic characteristics of human leukocyte antigen-DQB1 (HLA-DQB1) genotype in Hebei Han nationality population. Methods HLA-DQB1 high-resolution genotyping was performed by polymerase chain reaction- sequencing based typing (PCR-SBT) in 1 914 Hebei Han nationality population. The frequency of allele was calculated directly and compared with other Regions of Han nationality populations in China. Results 21 HLA-DQB1 alleles were detected from 1 914 Han nationality blood donors in Hebei, among which 6 were common alleles with frequency greater than 0.050 0, which were DQB1^*03∶01、DQB1^*03∶03、DQB1^*06∶02、DQB1^*02∶02、DQB1^*06∶01、DQB1^*05∶01. Conclusion DQB1 in Hebei Han nationality population is rich in polymorphism, and the distribution of genes has obvious regional characteristics. The knowledge of the distribution of HLA-DQB1 in Hebei Han nationality population will help to find the HLA-matched unrelated Hematopoietic stem cell donors and provide valuable basic information for population genetics research in China.

Key words: Hebei Han nationality population, HLA-DQB1, Allele, Gene frequency, Polymorphism