Abstract: ObjectiveTo investigate the relationship between anti-HCV, HCV RNA and ALT in blood donor volunteers. MethodsFluorescent quantitative PCR was used to detect viral loads and kinetic method was employed to assay ALT levels in 235 anti-HCV positive samples which had been tested by ELISA. A six months follow up was performed to monitor the anti-HCV(+)/HCV RNA(-) samples. ResultsAmong 235 anti-HCV positive samples, 140 (59.57%) were positive for HCV RNA. The HCV RNA positive rate in S/CO ratio 1~5 and 5.01~9.99 groups were 34.29% and 26.47%, respectively. The HCV RNA positive rate in S/CO ratio≥10 group was 81.68% and statistically significant differences were found between this and the other two groups(χ2=45.15,P <0.05;χ2=39.41,P<0.05 ).The ALT abnormal rates in anti-HCV(+)/HCV RNA(+) and anti-HCV(+)/HCV RNA(-) groups were 2.86% and 1.05%, respectively. No statistically significant difference was found (χ2=0.88,P≥0.05).The mean age of anti- HCV(+)/HCV RNA(+) donors was older than that of the anti-HCV(+)/HCV RNA(-) donors (χ2=7.81,P<0.05). Twenty three of 95 blood donors accepted retrospective survey, and one sample showed the negative anti-HCV in the second detection. ConclusionThe positive rate of HCV RNA is related to S/CO ratio of anti- HCV and the age of HCV infected blood donors. No correlation was found between the unqualified rate of ALT and HCV RNA in anti- HCV positive volunteer blood donors.

Key words: Volunteer, blood, donors, ALT, NAT