Abstract: Objective To compare the feasibility and validity of TRALI rat models induced by human plasma and rat homo/heterologous plasma respectively. Method After intraperitoneal injection of lipopolysaccharide in SD rats, SD rats, BN rats and human plasma were intravenously infused, and clinical symptoms, wet-dry ratio of lung tissue, and lung histopathological data were collected. Result All rats in the human plasma infusion group showed obvious TRALI-related clinical symptoms. Only 60% of the rats in the BN plasma infusion group showed different degrees of TRALI-related clinical symptoms. Almost none of the rats in the SD plasma infusion group showed TRALI-related clinical symptoms（P<0.05）. In addition, the wet-dry ratio of lung tissue and the pathological scores of lung injury in SD plasma group were significantly different from those in human plasma group（P<0.05）. There was significant difference in pathological scores of lung injury between BN plasma group and SD plasma group（P<0.05）. Conclusion Infusion of human plasma can effectively induce TRALI-SD-rat model with repeatability and stability.

Key words: Acute lung injury, Human, Rat, Homologous, Heterologous, Plasma