Abstract: Objective By analyzing irregular antibody screening of patients with blood transfusions,we explored its significance in blood transfusion safety and regional clinical guidance.Methods 38 722 patients with blood transfusions were studied from February 2012 to February 2016and among them positive irregular antibodies were screened and specificity was analyzed for safe and effective transfusion. Reasults 278 cases of positive irregular antibodies were found in the patients,which accounted for 0.717% （278/38 722）. Among them 83 males with a positive rate of 0.214% （83/38 722）,and 195 females with a positive rate of 0.503% （195/38 722）were observed. Female patients had a significantly high positive rate when compared with male patients（P<0.05）. The positive incidence of 0.114% （26/22 846）in the patients with history of blood transfusion and pregnancy was lower than that in control （1.587%,252/15 876）（P<0.05）.. . Among the 278 patients with irregular antibodies,128 （46.0%） patients presented specific antibodies,including 98 cases of RH （35.3%,98/278）,20 cases of MNS patients （7.2%,20/278）,and 10 cases of others（3.6%,10/278）. 150 （54%,150/278） patients had non-specific antibodies,,68 had cold antibodies （24.5%,68/278）,and 82（29.5%,82/278） carried other non-specific antibodies.Conclusion Analysis of the north central area of Guangxi province are similar with the other regions,under the normal conditions. Through the screening before blood transfusion,the patients with positive irregular antibodies should be analyzed for blood cross matchingand transfusion safety.

Key words: Irregular antibody, Clinical transfusion, Screening