Abstract: Objective To evaluate antineoplastic activity of granulocytes in blood donors screened by two different methods. Methods The anti-tumor genes were detected in 681 cases within 72 hours. The in vitro cytotoxicity assay of granulocytes and the anti-tumor gene test were carried out within 24 and 72 hours,respectively. 811 cases were detected. The results of immunoprecipitation were compared with those of 189 blood donors who had been tested for anti-tumor gene. The results were compared in 16 patients （excellent,good,moderate and low in 4 cases,respectively）. Blood donors were followed for 1 month and 3 months Of the gene detection and granulocyte in vitro killing test. Results The proportion of excellent blood donation was 8.4% and 11.0% was good. The excellent blood donation rate of granulocyte in vitro test was 19.9%,and the score of genetic testing was " Excellent "and" good "and 19.4% similar gene score and granulocyte in vitro killing test score roughly in line. Conclusion The result of genetic testing is stable,and the result is constant,which may be related to genetic information. The disadvantage is that it can not truly reflect the current anti-cancer activity of blood donors,and the cost of screening is high; the results of granulocyte in vitro killing test are unstable; The change of anticancer activity of the individual at different time may be related to the mood,physical condition and seasonal variation of blood donors. The advantage is that the screening cost is low and it is easy to be applied and popularized.

Key words: Donors, Neutropenia, High anticancer activity, Killer cells test, Cancer treatment