Abstract: Objective Peripheral blood stem cells （PBSCs）were collected in children donors by using COM.TEC autoMNC program to observe the changes of bone marrow mobilization,acquisition parameters,adverse reactions and haematology parameters. Methods PBSCs were collected from 53 donors,aged in 5.0±3.8 years and with body weight of 20.1±8.36 kg. The volume of anticoagulated whole blood was 4045.70±1167.30 ml,and 436.1±127.81 ml of anticoagulants and 213.21±59.88 minutes were used in the blood processing. Results Subcutaneous injection of 8-10μg/kg G-CSF was optimal in bone marrow mobilization（P=0.001）,showing a remarkable increase of peripheral granulocytes（P=0.01）.No significant difference of WBCs counts was noted but a decreased Hb and PLTs was seen in 53 cases （P<0.01）. No adverse reactions were observed in all cases. Conclusion The COM.TEC separator is acceptable and efficient for collecting PBSCs of juniors with no adverse reactions although a slight loss of Hb and PLTs might occur. Additionally,efficient mobilization of bone marrow cells and sedative status of the recipients are important in PBSCs collection.

Key words: Blood centrifuge, Children, Hematopoietic stem cells, Collecting, Peripheral blood cells collection