• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (1): 61-65.DOI: 10.3969/j.issn.1671-2587.2016.01.019

• 调查研究 • 上一篇    下一篇

福州地区无偿献血人群HCV基因型分布研究*

林洪铿,江伟梅,林授   

  1. 350004 福州,福建省血液中心,福建医科大学输血医学教研室
  • 收稿日期:2015-11-02 出版日期:2016-02-20 发布日期:2016-10-10
  • 作者简介:林洪铿（1977-）,男,福建莆田人,副主任技师,硕士,主要从事血液检测研究,（E-mail）linghongkeng@163.com。
  • 基金资助:
    *本课题受福建省卫生厅青年科研项目（No.2011-1-30)资助

The Genotype Distribution of HCV in Blood Donors in Fuzhou

LIN Hongkeng, JIANG Weimei, LIN Shou   

  1. Department of Blood Transfusion of Fujian Blood Center, Fujian Medical University, Fuzhou, Fujian, 35004
  • Received:2015-11-02 Online:2016-02-20 Published:2016-10-10

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摘要： 目的通过对血液筛查HCV阳性标本进行5′ UTR区扩增并测序,了解本地区无偿献血人群HCV感染的基因型分布特点。方法采用ELISA抗体检测试剂和TMA核酸检测试剂对献血者血液标本进行HCV筛查,对两者检测均为反应性的标本进行HCV基因5′ UTR区扩增并测序,再将测得序列通过构建进化树分型。结果ELISA法和核酸检测均为反应性的标本共92份,进行5′ UTR区基因成功扩增并测序52份,分型结果为：6a[(19/52)36.54%],1b[(18/52)34.62%],3a[(6/52) 11.54%],1a [(2/52)3.85%],3b[(2/52)3.85%],其他[(5/52)9.60%]。结论本地区无偿献血人群HCV感染的基因型主要以6a和1b为主。

关键词: 丙型肝炎病毒, 进化树, 基因型

Abstract: ObjectiveTo investigate the genotypes of HCV among blood donors in Fuzhou city of China. MethodsAntibodies against HCV and RNA were detected by ELISA and nucleic acid technology(NAT), and the possitive samples were amplified by PCR. The PCR-positive samples were subjected to 5′ UTR gene DNA sequencing, and HCV genotypes were determined by phylogenetic analysis. Results92 samples were both reactive by ELISA and NAT screening, and 52 of them were amplified successfully by PCR, all of these samples were determined the genotypes by sequencing 5’ UTR gene, and the results were 6a [(19/52)36.54%], 1b [(18/52)34.62%], 3a [(6/52) 11 .54%], 1a [(2/52)3.85%], 3b [(2/52)3.85%], others [(5/52)9.60%]. Conclusions6a and 1b were predominant HCV genotypes in the blood donors in Fuzhou city of China.

Key words: HCV, Phylogenetic, analysis, Genotype

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