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临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (6): 623-627.DOI: 10.3969/j.issn.1671-2587.2017.06.028

• 血型与临床 • 上一篇    下一篇

黑龙江省汉族人群血小板抗原1~17 系统基因多态性研究*

颜廷宇, 李鑫, 丁镌, 毕冬梅, 王鑫, 刘颖   

  1. 150056 黑龙江省哈尔滨市血液中心输血研究所
  • 收稿日期:2017-08-01 出版日期:2017-12-20 发布日期:2017-12-13
  • 通讯作者: 刘颖,女,主任技师,主要从事输血医学、免疫遗传学研究工作,（E-mail）liuying54609@126.com。
  • 作者简介:颜廷宇（1983-）,女,黑龙江拜泉人,主管技师,学士,主要从事输血医学、免疫遗传学研究工作,（Tel）0451-82437137。
  • 基金资助:
    *本课题受2012年度黑龙江省卫生厅科技计划课题（No.2012-081）资助

Genetic Polymorphism of HPA1-17 Alloantigen System in Heilongjiang Han Population

YAN Ting-Yu, Li Xin, Ding Juan, et al   

  1. Harbin Blood Center,Harbin 150056
  • Received:2017-08-01 Online:2017-12-20 Published:2017-12-13

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摘要： 目的 探讨黑龙江省汉族人群人类血小板同种抗原（Human platelet alloantigen,HPA）1~17系统基因多态性及其表达频率,确定有临床意义的血小板抗原系统。方法 选择123例汉族的健康无血缘关系的人群为研究对象,采用聚合酶链-序列特异性引物（PCR-SSP）技术,对HPA1-17系统等位基因进行分型,分别计算其基因频率、基因型频率,并与国内外不同人群进行比较。结果 黑龙江省汉族人群HPA-1a、2a、3a、5a、6a、15a 基因频率分别是0.987 8、0.959 4、0.682 8、0.971 5、0.983 7、0.479 6,HPA-4a、7a~14 a、16 a和17a均为1.0;HPA-1b 、2b 、3b、5b、6b 、15b 基因频率分别是0.012 2、0.040 7、0.317 1、0.028 5、0.016 3、0.520 4,未检测出HPA-4b、7b~14b、16b和17b。统计汉族人群HPA基因组合型及其频率,发现共有24种HPA基因组合型,前3种基因组合型频率均>10％（占55.28％）,其余21种基因组合型的频率均<7.5％（44.72％）。结论 黑龙江省汉族健康人群HPA1~17基因频率的分布与其他地区和国家相比具有本地区人群特点。HPA-3、15系统具有高度多态性,在随机血小板输注中,供受者HPA-3、15 系统不配合率分别为33.93％、37.46％,易发生同种免疫反应。

关键词: 人类血小板同种抗原, 基因型, 多态性

Abstract: Objective To analyze the allele frequencies and the polymorphisms of Human platlet antigen 1 to 17 system in Heilongjiang Han population for determination of their clinical significance. Method PCR-SSP was used for HPA1-17 genotyping among 123 unrelated Han nationality volunteer donors. The genotypes and gene frequencies of the each HPA gene system were calculated,and the difference with other populations were compared. Result The gene frequencies of HPA-1a,2a,3a,5a,6a,15a were 0.9878,0.9594 ,0.6828,0.9715,0.9837,and 0.4796,respectively,and the gene frequencies for all of HPA-4a,7a~14a,16a,17a were found to be 1.0. The gene frequencies of HPA-1b,2b,3b,5b,6b,15b were 0.0122,0.0407,0.3171,0.0285,0.0163,and 0.5204,respectively. HPA-4b and 7b to 14b and 16b to 17b were undetactabl. The study is the first time to investigate the assortment of HPA genes and their frequencies. The 24 assortment systems were found in Heilongjiang Han population,and only 3 of HPA gene frequencies more than 10%,and 21 less than 7.5% were noted. Conclusion Distribution of the allele polymorphisms of HPA 1-17 in Heilongjiang Han population was featured compared with other regions and countries. The system of HPA-3,15 in Han population with high polymorphism in that the incompatibility rates of donor recipient HPA-3,15 were 33.93％ and 37.46％ in random platelets infusion.

Key words: Human platelet antigens, Genotype, Polymorphism

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