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临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (2): 148-150.DOI: 10.3969/j.issn.1671-2587.2017.02.016

• 临床输血 • 上一篇    下一篇

97 835例献血者传染性指标检测结果的不重合性分析

王文婷, 胡兴斌, 孙文利, 张辉洁, 尤海菲, 杜姣, 尹文   

  1. 710032 陕西省西安市第四军医大学西京医院输血科（中国人民解放军西安血站）
  • 收稿日期:2016-12-01 出版日期:2017-04-20 发布日期:2017-04-24
  • 通讯作者: 尹文（1969-）,男,教授,博士生导师,主要从事免疫学研究工作,（E-mail）yinwen@fmmu.edu.cn。
  • 作者简介:王文婷（1984-）,女,陕西汉中人,主管技师,学士,主要从事血液检测工作,（Tel）13468834801。

Analysis of Divergent Epidemical Markers Detected by ELISA in 97 835 Volunteer Blood Donor

WANG Wen-ting, HU Xin-bin, SUN Wen-li, et al   

  1. Xi'an Blood Bank, Xijing Hospital,The Fourth Military University Xi'an 710032
  • Received:2016-12-01 Online:2017-04-20 Published:2017-04-24

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摘要： 目的 分析和探讨无偿献血者标本中传染性指标的2次ELISA法检测结果不重合的情况,为试剂选择及献血者筛选策略的制定提供依据。方法 选取本血站2013年1月~ 2015年11月97 835例无偿献血者标本,采用ELISA法检测HBsAg、抗-HCV、抗-HIV（1+2）和抗-TP四项传染性指标,对2次检测不合格标本中由单试剂检测不合格的结果进行统计分析。结果 97 835例标本中,抗-HIV（1+2）和抗-HCV的不重合率最高,抗-TP的不重合率最低。结论 试剂选择应进行筛选与组合,将不同试剂间不重合率的比对纳入试剂筛选的重要指标。

关键词: 传染性指标, ELISA, 不重合性

Abstract: Objective To investigate,and better the screen reagent as well recruitment strategy. To provide strong evidence for selecting high quality reagent and recruitment strategy,we analyzed whether twice ELISA results of pathogen marker detection in blood donors was consistent.Methods ELISA was performed to detect HBsAg,anti-HCV,anti-HIV（1+2） and anti-TP,in 97 835 blood donor samples collected from Jan. 2013 to Nov. 2015. Statistical analysis was employed to evaluate the divergent rate of each pathogen marker in the discharged donations.Results The results showed that there was higher inconsistent rate in anti-HIV （1+2） and anti-HCV detection,while the lowest inconsistent rate was in anti-TP detection.Conclusion sReagents for ELISA would be selected and grouped,thus the inconsistent detection data should be taken into account to select ELISA kit.

Key words: Epidemical marker, ELISA, Noncoincidence

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