• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2019, Vol. 21 ›› Issue (2): 162-165.DOI: 10.3969/j.issn.1671-2587.2019.02.014

• 血液检测 • 上一篇    下一篇

ELISA法检测抗-HIV灰区设置的探讨

王桂喜, 汪道法   

  1. 244000 安徽省铜陵市中心血站
  • 收稿日期:2018-11-20 出版日期:2019-04-20 发布日期:2019-04-10
  • 作者简介:王桂喜（1968-）,男,安徽界首人,副主任技师,学士,主要从事血站血液检测工作,（Tel）13645622083（E-mail）553972529@qq.com。

Determination of the Grey Zone of ELISA in Anti-HIV Detections

WANG Gui-xi, WANG Dao-fa   

  1. Tongling Red Cross Blood Station,Tongling,244000
  • Received:2018-11-20 Online:2019-04-20 Published:2019-04-10

PDF

1682

摘要： 目的 探讨采用ELISA法对无偿献血者进行抗-HIV筛查所设灰区的合理性。方法 回顾分析本站2014年1月1日~2017年12月31日无偿献血者抗-HIV有反应性和灰区（0.5≤s/co<1）标本,以市疾病控制中心对其确认结果作为金标准,绘制ROC曲线,比较不同s/co情况下试剂灵敏度和特异性的变化。随机抽取20份抗-HIV非反应性标本（s/co≤0.5）做WB确认,确定是否有阳性标本漏检。结果 万泰试剂对39 991标本检测出有反应性标本26份,灰区22份;丽珠试剂检测出有反应性15份,灰区标本7份;市疾病控制中心共确认5例为阳性;所有灰区标本经确认均阴性;20例无反应性标本确认均阴性。结论 试剂厂商提供的s/co值具有较高灵敏度,满足血液筛查要求,但是设置的灰区造成不必要的血液浪费。

关键词: 灰区, ROC曲线, 抗-HIV, ELISA

Abstract: Objective To explore the rationality of the grey zone determination in anti-HIV detections by ELISA in screening of blood donation. Method sA retrospective analysis was performed with anti-HIV positive and grey zone (0.5≤s/co<1) specimens that were subsequently subjected to Tongling Center for Disease Control for confirmation from January 2014 to December 2017. The ROC curve was used to compare the sensitivity and specificity of the reagents under different s/co conditions. Twenty negative specimens of anti-HIV(s/co<0.5 ) were randomly selected for WB tests. Results A total of 39991 specimens were tested by the reagents from Wantai company,and 26 positive specimens and 22 grey zone specimens were found. Fifteen positive and 7 grey zone specimens were detected out by the reagents provided by Lizhu company. Among them,however,only 5 specimens were confirmed to be positive by Tongling CDC. Conclusion The over-sensitive values of s /co recommended by the reagent manufacturers for blood screening is able to meet our requirements and the setting of grey zone may cause unnecessary waste of donated blood.

Key words: Grey, zone, ROC, curve, Anti-HIV, ELISA

中图分类号: