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临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (5): 505-507.DOI: 10.3969/j.issn.1671-2587.2017.05.029

• 技术与方法 • 上一篇    下一篇

ELISA检测抗-HCV灰区、弱反应标本与FQ-PCR检测结果对比分析

邹宵萌   

  1. 473000 河南省南阳市南石医院输血科
  • 收稿日期:2017-02-18 出版日期:2017-10-20 发布日期:2017-10-26
  • 作者简介:邹宵萌（1971-）,女,河南南阳人,主管技师,主要从事临床输血和检验工作,（Tel）13838619609（E-mail）3266628713@qq.com。

A Compararison of ELISA with FQ-PCR for Detection of Anti-HCV Antibodies in the Samples Near Critical Value

ZOU Xiao-meng   

  1. Department of Blood Transfusion,Nanyang Nanshi Hospital,Nanyang,473000
  • Received:2017-02-18 Online:2017-10-20 Published:2017-10-26

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摘要： 目的 探讨丙型肝炎病毒抗体（抗-HCV）酶联免疫吸附试验（ELISA）检测呈灰区、弱阳性样本采用荧光定量PCR（FQ-PCR）的复检情况。方法 选取2012年4月~2016年3月于本院手术和输血治疗的1 348例患者为研究对象,根据ELISA法抗-HCV检测结果将患者标本分为：A组：阴性（S/CO<0.3）240例;B组：灰区（0.7≤S/CO<1）902例;C组：弱反应性（1≤S/CO<3.8）206例,所有研究对象均采用FQ-PCR复检,比较两种方法检测结果。结果 B组灰区和C组弱反应性标本中,FQ-PCR复检分别有41例（4.54%）和172例（83.49%）HCV RNA浓度>1 000 IU/ml;ELISA检测抗-HCV双试剂灰区HCV RNA FQ-PCR阳性率高于单试剂灰区,差异有统计学意义（P<0.05）;ELISA检测抗-HCV双试剂弱反应性HCV RNA FQ-PCR阳性率与单试剂弱反应性比较,差异无统计学意义（P>0.05）。结论 ELISA法检测抗-HCV为灰区、弱反应性标本存在一定的HCV RNA阳性标本漏检和误诊,采用FQ-PCR检测对于HCV感染早期诊断和治疗尤为重要。

关键词: ELISA, 弱反应性, 抗-HCV, FQ-PCR

Abstract: Objective To Use the fluorescence quantitative PCR （FQ-PCR） for confirming antibodies against hepatitis C surface antigen（anti-HCV）that had been tested by enzyme linked immunoassay（ELISA）. Methods 1 348 patients who had experienced blood transfusion and operation were collected from April 2012 to March 2016.The patients were divided into three groups,group A included 240 samples with negative anti-HCV detected by ELISA （S/CO<0.3）; group B covered 902 samples with anti-HCV on cutoff value （0.7≤S/CO<1）; and group C included 206 samples with weak reaction of anti-HCV detected by ELISA. Results Among groups B and C,41（4.54%）and 172（83.49%） samples were found to have HCV RNA level>1 000 IU/ml rechecked by FQ-PCR. FQ-PCR demonstrated a high positive rate in the samples on the cutoff value tested by ELISA double reagents（P<0.05）; while no difference was noted between the samples with weak reaction of HCV RNA when tested with ELISA and FQ-PCR（P>0.05）. Conclusion The false negative and misdiagnosis rate of ELISA is high in the samples near HCV cutoff value and in weak reaction,suggesting that FQ-PCR is useful for early diagnose of HCV patients.

Key words: ELISA, Weak reactive, Anti-HCV, FQ-PCR

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