• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (4): 368-371.DOI: 10.3969/j.issn.1671-2587.2017.04.017

• 临床检验 • 上一篇    下一篇

血液分析仪散点图在分析嗜碱性粒细胞假性升高中的应用*

姚丽娟, 罗以勤, 王力, 孙余会, 黄凤萍, 李丽   

  1. 230001 合肥,安徽省立医院检验科
  • 收稿日期:2017-02-19 出版日期:2017-08-20 发布日期:2017-08-09
  • 作者简介:姚丽娟（1978-）,女,安徽庐江人,主管检验师,硕士,主要从事临床检验、分子生物学诊断,（E-mail）chinavip166@163.com。
  • 基金资助:
    *本课题受安徽省自然科学基金赞助项目（No.1608085-MH229）资助

Application of Scatter Diagram of Hematology Analyzer to Survey of False Increase of Basophil Count

Yao Li-juan, Luo Yi-qin, Wang Li, et al   

  1. Department of Laboratory Medicine,the Affiliated Provincial Hospital of Anhui Medical University,Hefei 230001
  • Received:2017-02-19 Online:2017-08-20 Published:2017-08-09

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摘要： 目地 分析白细胞分类通道（DIFF）和嗜碱性粒细胞通道（WBC/BASO）散点图的典型变化,判断嗜碱性粒细胞（Baso）假性升高,探讨其临床应用价值。方法 收集46例住院患者经手工涂片复检确认的Baso假性升高样本的白细胞分类数据信息和散点图,分析导致假性升高的原因及归纳典型散点图变化。结果 Baso假性升高干扰主要来源于异型淋巴细胞（Abn-L）、中性中毒颗粒（TG）和采血不当3种原因,以Abn-L干扰较常见（56.5%）。Baso假性升高具有典型Abn-L或TG的DIFF和WBC/BASO散点图者,经外周血涂片镜检均可检出异淋或TG,白细胞分类计数（DC）发现异常增高的部分均来源于Abn-L或TG;当具有正常DIFF图,异常WBC/BASO典型采血不当散点图时,外周血涂片镜检分类均正常,临床重新规范抽取样本送检后结果均正常。结论 对于外周血Baso计数存在假性升高的样本,实验室可通过仪器提供的典型散点图快速判断升高来源并指导复检,为临床提供准确可靠的实验数据。

关键词: 嗜碱性粒细胞, 散点图, 嗜碱性粒细胞计数

Abstract: Objective To analyze the change of the scatter plots of white blood cell and basophilic granulocyte count channels （WBC/BASO）and explore the resean for false elevation of basophils count. Methods The data of scatter diagram of false elevation in Baso counts were collected that had been verified by manual counting in blood smears of 46 impatients. Results Three factors contributed to the false increase of Baso count,including abnomal lymphocyte （Abn-L）,neutrophil toxic granules （TG）and improper blood collection. Among them,Abn-L interference dominates（56.5%）. All of the samples of false elevated Baso counts with typical differential luekocye counts and Abn-L or TG WBC/Baso scatter diagram presented abnormal lymphocyte count or TG by manual counting in periphera blood smears. Differential leukocyte counting （DC）showed that the abnormal increase was originated from Abn-L or TG. Conclusion The samples with false increase of Baso count should be comfirmed by instrumental typical scatter diagram to provide orientation of repeated detection and correct data for clinical use.

Key words: Basophil, Scatter diagram, Basophil count

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