SENEX基因对急性髓系白血病细胞增殖和凋亡的影响*

doi:10.3969/j.issn.1671-2587.2022.02.017

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (2): 222-225.DOI: 10.3969/j.issn.1671-2587.2022.02.017

SENEX基因对急性髓系白血病细胞增殖和凋亡的影响^*

王佳, 陶千山, 沈元元, 董毅

230601 安徽医科大学第二附属医院血液科

收稿日期:2021-12-15 发布日期:2022-04-12
通讯作者: 董毅,男,主任医师,主要研究方向为肿瘤免疫与免疫治疗方面研究,（E-mail）dongyixx@126.com。
作者简介:王佳（1988-）,女,安徽无为人,主治医师,博士,主要从事肿瘤免疫与免疫治疗方面研究,（E-mail）wangjia198@126.com。
基金资助:
*本课题受国家自然科学基金（No. 81401293）; 安徽省重点研究与开发计划项目（No. 202104j07020030）资助

Effect of SENEX on the Proliferation and Apoptosis of AML Cells

WANG Jia, TAO Qian-shan, SHEN Yuan-yuan, et al

Department of Hematology, the Second Hospital of Anhui Medical University, Anhui, Hefei 230601

Received:2021-12-15 Published:2022-04-12

摘要/Abstract

摘要： 目的探讨SENEX基因对急性髓细胞性白血病（acute myeloid leukemia,AML）细胞增殖和凋亡的影响。方法选取20例成人初诊AML患者,5例复发AML,17例完全缓解AML以及7例对照的骨髓标本,qPCR检测SENEX基因的表达水平,流式细胞术检测细胞凋亡,CCK8检测细胞增殖。结果（1）SENEX基因在初诊及复发AML中表达水平升高,且和疾病状态相关;（2）干扰SENEX基因后,NB4细胞的细胞增殖效率下降,凋亡比例上升;（3）IL-35可减弱阿糖胞苷诱导的NB4细胞凋亡比例及SENEX基因上升趋势。结论 SENEX基因可通过直接促进AML细胞增殖和抑制AML细胞的凋亡来参与AML的发生发展。

关键词: 白血病, 急性髓系白血病, SENEX基因

Abstract: Objective To investigate the effect of SENEX on the proliferation and apoptosis of Acute Myeloid Leukemia (AML) cells. Methods 20 adult patients with newly diagnosed AML, 5 relapsed AML, 17 complete remission of AML, and 7 control bone marrow samples were selected. The expression level of SENEX was detected by qPCR, apoptosis was detected by flow cytometry, and cell proliferation was detected by CCK8. Results (1) The expression level of SENEX increased in newly diagnosed and relapsed AML, and was related to the disease status; (2) After sliencing with SENEX, the cell proliferation efficiency of NB4 cells decreased and the proportion of apoptosis increased; (3) IL-35 can attenuate the apoptotic proportion of NB4 cell and the upward trend of SENEX gene induced by cytarabine . Conclusions enex might participate in the occurrence and development of AML by directly promoting the proliferation of AML cells and inhibiting the apoptosis of AML cells.

Key words: Leukemia, Acute myeloid leukemia, SENEX

中图分类号:

R733.71

王佳, 陶千山, 沈元元, 董毅. SENEX基因对急性髓系白血病细胞增殖和凋亡的影响^*[J]. 临床输血与检验, 2022, 24(2): 222-225.

WANG Jia, TAO Qian-shan, SHEN Yuan-yuan, et al. Effect of SENEX on the Proliferation and Apoptosis of AML Cells[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(2): 222-225.

参考文献

[1] NEWELL L F,COOK R J.Advances in acute myeloid leukemia[J]. BMJ,2021,375:n2026.
[2] DINARDO C D,SCHUH A C,STEIN E M,et al.Enasidenib plus azacitidine versus azacitidine alone in patients with newly diagnosed,mutant-IDH2 acute myeloid leukaemia (AG221-AML-005):a single-arm,phase 1b and randomised,phase 2 trial[J]. Lancet Oncol,2021,22(11):1597-1608.
[3] RAVANDI F,ROBOZ G J,WEI A H,et al.Management of adverse events in patients with acute myeloid leukemia in remission receiving oral azacitidine:experience from the phase 3 randomized QUAZAR AML-001 trial[J].J Hematol Oncol,2021,14(1):133.
[4] KATOH M,KATOH M.Characterization of human ARHGAP10 gene in silico[J].Int J Oncol,2004,25(4):1201-1206.
[5] 完颜智翔,王极宇,潘莹,等.SENEX基因通过活化Rb/E2F通路促进弥漫大B细胞淋巴瘤增殖与复发[J].安徽医科大学学报,2020,55(3):432-437.
[6] 曹艳芸,陶千山,王会平,等.senex基因在急性髓系白血病中的表达及意义[J].安徽医科大学学报,2017,52(3):401-405.
[7] 张之南,沈悌. 血液病诊断及疗效标准[M]. 第3版. 北京:科学出版社,2007:106-115.
[8] 王佳,陶千山,翟志敏.IL-35对急性髓系白血病细胞增殖和凋亡的影响[J].锦州医科大学学报,2017,38(5):1-4.
[9] FLEISCHMANN M,SCHNETZKE U,HOCHHAUS A,et al.Management of acute myeloid leukemia:current treatment options and future perspectives[J]. Cancers,2021,13(22):5722.
[10] CHEN T P,WANG H P,ZHANG Z Q,et al.A novel cellular senescence gene,SENEX,is involved in peripheral regulatory T cells accumulation in aged urinary bladder cancer[J]. PLoS One,2014,9(2):e87774.
[11] WANG J Y,TAO Q S,PAN Y,et al.Stress-induced premature senescence activated by the SENEX gene mediates apoptosis resistance of diffuse large B-cell lymphoma via promoting immunosuppressive cells and cytokines[J]. Immun Inflamm Dis,2020,8(4):672-683.
[12] WANG J Y,WANG Z T,WANG H P,et al.Stress-induced premature senescence promotes proliferation by activating the SENEX and p16 INK4a/retinoblastoma (Rb) pathway in diffuse large B-cell lymphoma[J]. Turk J Haematol,2019,36(4):247-254.
[13] CHEN T P,WANG H P,ZHANG Z Q,et al.A novel cellular senescence gene,SENEX,is involved in peripheral regulatory T cells accumulation in aged urinary bladder cancer[J]. PLoS One,2014,9(2):e87774.
[14] JIA X Y,YANG W Z,ZHOU X H,et al.Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome[J]. Oncol Lett,2020,19(1):442-448.

[1]	刘琴, 徐春芬, 宋婕, 谢松丽, 陈涵薇, 胡红兵. 输注不同治疗剂量血小板在56例急性淋巴细胞白血病患儿中的临床观察^*[J]. 临床输血与检验, 2022, 24(2): 218-221.
[2]	杨世礼, 孙晓星, 楚海亮, 王其凯, 李古郡, 葛洪峰. 两种方案对复发难治性急性髓系白血病患者疗效及预后的影响[J]. 临床输血与检验, 2022, 24(1): 97-103.
[3]	向鑫, 刘恒芳, 曾招, 白淑潇, 岑建农, 金松, 沈宏杰, 陈苏宁, 潘金兰. 三例伴有t（18;22）（q21;q11）慢性淋巴细胞白血病的细胞及分子遗传学研究^*[J]. 临床输血与检验, 2021, 23(4): 473-478.
[4]	尹石红, 徐修才. MiR-149通过PHGDH对慢性髓系白血病细胞葡萄糖代谢的影响^*[J]. 临床输血与检验, 2021, 23(3): 294-300.
[5]	张佳思, 谭方方, 袁嘉, 符刚, 阮潜瑛, 姜盛, 黎诗琦, 钱程. CAR-T治疗急性白血病患者严重并发症CRS与CRES救治的规范化护理^*[J]. 临床输血与检验, 2021, 23(3): 384-388.
[6]	王慧茹, 刘会兰. 非血缘脐血移植术后患者输血情况相关影响因素分析^*[J]. 临床输血与检验, 2021, 23(2): 162-166.
[7]	施岚, 许良飞, 聂正超, 常文娇, 马筱玲. 金葡菌杀白细胞素S组分LukS-PV抑制急性髓系白血病细胞增殖的机制研究^*[J]. 临床输血与检验, 2020, 22(6): 603-609.
[8]	吴月, 朱小玉, 刘会兰, 宋闿迪, 汤宝林, 孙自敏. 强化清髓单份非血缘脐血移植治疗FLT3-ITD突变阳性急性髓系白血病疗效观察^*[J]. 临床输血与检验, 2020, 22(6): 627-632.
[9]	强萍, 薛磊, 汪敏, 许汉英, 刘欣, 朱薇波, 杨林, 王兴兵. 成功救治2例CAR-T细胞治疗后伴严重细胞因子风暴和相关性脑病伴文献复习[J]. 临床输血与检验, 2020, 22(4): 413-419.
[10]	沈季敏, 刘欣, 王兴兵. TLR-3激动剂联合TLR-9激动剂对树突状细胞细胞形态及免疫表型的影响*[J]. 临床输血与检验, 2020, 22(1): 71-76.
[11]	黎艳湘, 邸玉玮, 陈倩礼, 谢佳斌. 白血病细胞对外周血钾、葡萄糖检测的影响^*[J]. 临床输血与检验, 2019, 21(6): 641-645.
[12]	吴金菊, 郭青, 刘欣, 王兴兵, 汪健, 李庆, 沈季敏. 去甲氧柔红霉素和硼替佐米对急性髓系白血病细胞凋亡及钙网蛋白表达的影响[J]. 临床输血与检验, 2019, 21(5): 502-505.
[13]	王原媛, 李莉, 刘方方. 白血病抑制因子及其受体在原发性不孕症患者子宫内膜中的表达分析^*[J]. 临床输血与检验, 2019, 21(2): 166-169.
[14]	李丽, 崔成华, 徐建宁, 艾晓非, 王建祥, 李承文. 21例伴t（11;19）（q23;p13）白血病的临床和实验室特征[J]. 临床输血与检验, 2019, 21(1): 87-92.
[15]	李莉华, 戚海, 陈莉, 马印图. 石家庄地区无偿献血者HTLV-Ⅰ/Ⅱ检测情况分析[J]. 临床输血与检验, 2018, 20(5): 482-486.

SENEX基因对急性髓系白血病细胞增殖和凋亡的影响^*

Effect of SENEX on the Proliferation and Apoptosis of AML Cells

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

在线期刊

访问统计

联系我们