关键词: 血小板, 黏附, 血小板聚集, 流动小室

Abstract: Objective Develop a suitable in vitro model to observe the adhesion of platelets on the vessel wall under pathological vascular conditions（including stenosis,blood flow disturbance,venous valve damage,etc.）. Methods In this study,an improved parallel flow chamber device was designed to detect the adhesion of platelets under physiological and pathological conditions with different shear rates and different vessel diameters. At the same time,the aggregation of platelets that flow through the chamber but not adhered was measured. Results Peripheral blood with fluorescently labeled platelets flows through the collagen-coated flow chamber at a normal flow rate. Platelets adhered to the tube wall can be detected in about 5~8 minutes. The maximum platelet coverage area is about 60%; the inhibitor SZ-2 can significantly inhibit platelet adhesion. The same platelet adhesion area increases in the local stenosis lumen and the lumen with blood vessel branches; the platelet aggregation rate flowing through the local stenosis lumen is significantly higher than that of the platelets flowing through the normal lumen. Conclusion The improved parallel flow chamber can simulate the blood flow state under physiological and vascular pathological conditions,and has application value for researching platelet function and monitoring the efficacy of antithrombotic reagents.

Key words: Platelet, Adhesion, Aggregation, Flow chamber