X射线辐照对淋巴细胞的灭活能力及对悬浮红细胞质量的影响研究

doi:10.3969/j.issn.1671-2587.2023.03.006

临床输血与检验 ›› 2023, Vol. 25 ›› Issue (3): 320-323.DOI: 10.3969/j.issn.1671-2587.2023.03.006

X射线辐照对淋巴细胞的灭活能力及对悬浮红细胞质量的影响研究

陈怡静, 卓海龙, 张怡, 程晨, 骆群

100071 解放军总医院第五医学中心

收稿日期:2023-04-24 发布日期:2023-07-10
通讯作者: 骆群,主任医师,博士,主要从事临床输血研究,（E-mail）123498896@qq.com。
作者简介:陈怡静,主要从事临床输血研究,（E-mail）1822724692@qq.com。

Effect of X-ray Irradiation on the Inactivation Ability of Lymphocytes and the Quality of Suspended Blood Cells

CHEN Yijing, ZHUO Hailong, ZHANG Yi, et al

The Fifth Medical Center of PLA General Hospital,Beijing 100071

Received:2023-04-24 Published:2023-07-10

摘要/Abstract

摘要： 目的探讨X射线辐照对悬浮红细胞中淋巴细胞灭活能力及辐照后14 d内对红细胞质量的影响。方法将悬浮红细胞分为三组：X射线辐照组、γ射线辐照组、未辐照组,辐照组分别使用X射线辐照仪、γ射线辐照仪以25 Gy剂量辐照。分离上述三组悬浮红细胞的淋巴细胞进行培养,比较两种辐照方法对悬浮红细胞中淋巴细胞的灭活效果;检测辐照后第0、7、14 d悬浮红细胞的溶血率、2,3-DPG、ATP、红细胞渗透脆性等。结果 X射线辐照、γ射线辐照后淋巴细胞增殖抑制率分别为（98.034±1.778）%和（97.882±1.915）%,且两组在辐照后14 d内溶血率、2,3-DPG、ATP、红细胞渗透脆性等差异均无统计学意义（P>0.05）。结论 X射线血液辐照仪和γ射线辐照仪对淋巴细胞的灭活效果无明显差异,且25 Gy辐照对悬浮红细胞质量无明显影响。

关键词: X射线, γ射线, 悬浮红细胞, 淋巴细胞, 输血相关移植物抗宿主病

Abstract: Objective To investigate the effect of X-ray irradiation on the inactivation ability of lymphocytes in suspended red blood cells and the quality of red blood cells within 14 days after irradiation. Methods The suspended red blood cells were divided into three groups: X-ray irradiation group, γ-ray irradiation group and non-irradiation group. The irradiation group was irradiated with 25Gy X-ray and γ-ray irradiation instrument respectively. Lymphocytes from the three groups of suspended red blood cells were isolated and cultured to compare the inactivation effects of the two irradiation methods on lymphocytes in suspended red blood cells. The hemolysis rate, 2,3-DPG, ATP and osmotic fragility of red blood cells at 0, 7 and 14 days before and after irradiation were detected. Results The inhibitory rates of lymphocyte proliferation after X-ray irradiation and γ-ray irradiation were (98.034±1.778) % and (97.882±1.915) % respectively, and there were no significant differences in hemolysis rate, 2,3-DPG, ATP and osmotic fragility of erythrocytes between the two groups within 14 days after irradiation (P>0.05). Conclusion There is no significant difference in the inactivation effect of lymphocyte between X-ray and γ-ray irradiation, and 25Gy irradiation has no significant effect on the quality of suspended red blood cells.

Key words: X-rays, γ-rays, Suspended red blood cells, Lymphocytes, Transfusion-associated, Graft-versus-host disease

中图分类号:

R457

陈怡静, 卓海龙, 张怡, 程晨, 骆群. X射线辐照对淋巴细胞的灭活能力及对悬浮红细胞质量的影响研究[J]. 临床输血与检验, 2023, 25(3): 320-323.

CHEN Yijing, ZHUO Hailong, ZHANG Yi, et al. Effect of X-ray Irradiation on the Inactivation Ability of Lymphocytes and the Quality of Suspended Blood Cells[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(3): 320-323.

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X射线辐照对淋巴细胞的灭活能力及对悬浮红细胞质量的影响研究

Effect of X-ray Irradiation on the Inactivation Ability of Lymphocytes and the Quality of Suspended Blood Cells

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