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临床输血与检验

临床输血与检验 ›› 2024, Vol. 26 ›› Issue (6): 777-780.DOI: 10.3969/j.issn.1671-2587.2024.06.011

• 调查研究 • 上一篇    下一篇

上海地区无偿献血人群HLA抗体筛查分析

蔡茵, 陈至颖, 蒋苓, 任亚娜, 郑岚, 周国平   

  1. 上海市血液中心,上海 200051
  • 收稿日期:2024-05-29 出版日期:2024-12-20 发布日期:2024-12-20
  • 通讯作者: 周国平,主要从事血液筛查实验室质量管理及相关研究,（E-mail）zhouguoping@sbc.org.cn。
  • 作者简介:蔡茵,主要从事血液筛查检测相关研究,（E-mail）caiyin@sbc.org.cn。

Screening Analysis of HLA Antibodies of Blood Donors in Shanghai Area

CAI Yin, CHEN Zhiying, JIANG Ling, REN Yana, ZHENG Lan, ZHOU Guoping   

  1. Shanghai Blood Center, Shanghai 200051
  • Received:2024-05-29 Online:2024-12-20 Published:2024-12-20

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摘要： 目的 了解上海地区无偿献血人群HLA抗体发生率,为输血相关急性肺损伤（TRALI）研究提供数据。方法 随机选取2020年10月—2021年4月的无偿献血者标本,采用流式细胞仪-微珠法进行HLA特异性抗体检测,并统计分析HLA抗体发生率。结果 在9 797例无偿献血者样本中发现HLA抗体阳性1 715例（17.51%）,其中男性、女性HLA抗体发生率分别为4.81%、28.08%。排除输血史,无妊娠史女性、有妊娠史女性HLA抗体发生率分别为12.10%、36.62%,分组比较妊娠1次、2次、3次及以上人群,HLA抗体阳性率分别为29.97%、40.70%、44.80%。多次妊娠史女性献血者HLA抗体发生率明显高于单次妊娠献血者（P<0.05）。结论 血站开展女性献血者孕产史征询和HLA抗体检测,可预防TRALI的发生。

关键词: 献血者, HLA-Ⅰ类抗体, HLA-Ⅱ类抗体, HLA-Ⅰ+HLA-Ⅱ类抗体, 输血相关急性肺损伤

Abstract: Objective To know the frequency of HLA antibodies in donors in Shanghai area, to provide basic data for the research on TRALI. Methods Samples of blood donors from October 2020 to April 2021 were randomly selected to detect HLA-specific antibodies by flow cytometry and microbead method, and the incidence of HLA antibodies was statistically analyzed. Results In 9 797 serum samples of blood donors, 1 715 (17.51%) were positive for HLA antibodies, among which the frequency of HLA antibodies in males and females was 4.81% and 28.08%. Excluding the history of blood transfusion, the frequency of HLA antibodies in women with no pregnancy history and women with pregnancy history was 12.10% and 36.62%, and comparative analysis the group of once, twice and three or more times pregnancies donors population, which frequency of HLA antibodies was 29.97%, 40.70% and 44.80%. The frequency of HLA antibodies in female donors with multiple pregnancy history was significantly higher than that in single pregnancy (P<0.05). Conclusion Consultation of pregnancy history and HLA antibodies detection of female blood donors in blood stations can prevent from the occurrence of TRALI.

Key words: Blood donors, HLA ClassⅠantibody, HLA Class Ⅱ antibody, HLA ClassⅠand Ⅱ antibodies, Transfusion-related acute lung injury

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