试剂抗体对输血相容性检测试验影响的探讨研究

doi:10.3969/j.issn.1671-2587.2026.03.011

临床输血与检验 ›› 2026, Vol. 28 ›› Issue (3): 363-368.DOI: 10.3969/j.issn.1671-2587.2026.03.011

试剂抗体对输血相容性检测试验影响的探讨研究

许志远, 李晓菲, 刘婷婷, 郑仲男, 苗天红

北京市红十字血液中心,北京 100088

收稿日期:2026-03-18 出版日期:2026-06-20 发布日期:2026-07-07
作者简介:许志远,主要从事血型血清学和分子生物学研究,（E-mail）zhiyuanxxu@sina.com。

Impacts of Reagents-dependent Antibodies on Blood Transfusion Compatibility Testing

XU Zhiyuan, LI Xiaofei, LIU Tingting, ZHENG Zhongnan, MIAO Tianhong

Beijing Red Cross Blood Center, Beijing 100088

Received:2026-03-18 Online:2026-06-20 Published:2026-07-07

摘要/Abstract

摘要： 目的探讨试剂抗体对输血相容性检测的影响和解决策略。方法对5例临床医院送检的疑似产生试剂抗体样本,进行ABO血型鉴定、抗体筛查、抗体鉴定、交叉配血,方法包括试管抗人球蛋白法和卡式抗人球蛋白法。结果标本1血浆中存在针对红细胞悬浮介质抗体,标本2血浆中存在针对反定型细胞悬浮介质的试剂抗体,标本3血浆中存在针对增强介质Liss的试剂抗体,标本4血浆中存在针对增强介质Liss的试剂抗体,且具有类似抗-C、e特异性,标本5血浆中存在柱基质试剂抗体。结论通过综合分析、比较不同试剂、反应介质、检测方法对于同一患者标本的不同结果,可准确识别试剂抗体。

关键词: 试剂抗体, 抗体筛查, 抗体鉴定, 安全输血

Abstract: Objective To investigate the influence of reagent-dependent antibodies on blood transfusion compatibility testing and to explore potential resolution strategies. Methods Five difficult suspected reagent-dependent antibody generated patient samples referred by clinical hospitals were analyzed. ABO blood grouping, antibody screening, antibody identification and crossmatching were performed using the in vitro anti-human globulin method and the anti-human globulin card method. Results Sample 1 had antibodies against the red blood cell suspension medium, Sample 2 exhibited reagent antibodies against the anti-typing cell suspension medium, Sample 3 displayed reagent antibodies against the enhancer medium Liss, and Sample 4 also had reagent antibodies against the enhancer medium Liss, both showing similar anti-C and anti-e specificityies. Samples 5 had antibodies against the albus reagent. Conclusion By conducting a comprehensive analysis and comparing the different results obtained from different reagents, reaction media, and detection methods for the same patient sample, the specific reagent-denpendent antibody can be accurately identified.

Key words: Reagent-dependent antibody, Antibody screening, Antibody identification, Blood transfusion safety

中图分类号:

R457.1

许志远, 李晓菲, 刘婷婷, 郑仲男, 苗天红. 试剂抗体对输血相容性检测试验影响的探讨研究[J]. 临床输血与检验, 2026, 28(3): 363-368.

XU Zhiyuan, LI Xiaofei, LIU Tingting, ZHENG Zhongnan, MIAO Tianhong. Impacts of Reagents-dependent Antibodies on Blood Transfusion Compatibility Testing[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(3): 363-368.

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试剂抗体对输血相容性检测试验影响的探讨研究

Impacts of Reagents-dependent Antibodies on Blood Transfusion Compatibility Testing

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