一例IgM+IgG型抗-K抗体鉴定与分析

doi:10.3969/j.issn.1671-2587.2026.03.020

临床输血与检验 ›› 2026, Vol. 28 ›› Issue (3): 425-429.DOI: 10.3969/j.issn.1671-2587.2026.03.020

一例IgM+IgG型抗-K抗体鉴定与分析

康世蓉, 王丹, 袁小华, 陆红芹, 李苏亮

西安医学院第一附属医院,陕西西安 710000

收稿日期:2025-10-28 出版日期:2026-06-20 发布日期:2026-07-07
通讯作者: 李苏亮,主要从事输血医学,血液管理、输血治疗相关研究,（E-mail）lisuliang01@163.com。
作者简介:康世蓉,主要从事输血医学及基础医学研究、教学及科研工作,（E-mail）18700997031@163.com。

A Case of Mixed IgM and IgG Anti-K Antibody: Identification and Clinical Implications

KANG Shirong, WANG Dan, YUAN Xiaohua, LU Hongqin, LI Suliang

The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710000

Received:2025-10-28 Online:2026-06-20 Published:2026-07-07

摘要/Abstract

摘要： 目的对一例不规则抗体筛查阳性患者血清中发现的抗-K抗体进行鉴定,测定抗体的性质及效价,并分析产生的原因。方法采用血清学方法检测患者的ABO血型及Rh分型;采用盐水介质试管法（NS）、微柱凝胶法（MGT）进行不规则抗体筛查及鉴定;交叉配血采用NS、MGT及手工凝聚胺法（MPT）进行;采用2-巯基乙醇（2-Me）试剂处理患者血清测定抗体的性质及效价。结果患者血清不规则抗体筛查为阳性,特异性鉴定为抗-K抗体,性质为IgM+IgG型,其中IgM型效价为2,IgG型效价为256。结论利用合适的实验室检测方法可以准确鉴定出血清中存在的IgM+IgG型抗-K抗体,为临床输血提供安全保障。

关键词: Kell血型, 抗-K抗体, 抗体鉴定, IgM抗体, IgG抗体

Abstract: Objective To identify the specificity, immunoglobulin subclass and titer of an anti-K antibody detected in a patient with a positive irregular antibody screen, and to analyze potential etiological factors contributing to its formation. Methods ABO blood grouping and Rh phenotyping were performed using standard serological methods. Irregular antibody screening and identification were carried out by normal saline (NS) tube test and microcolumn gel test (MGT). Crossmatching was conducted using NS, MGT, and manual polybrene test (MPT). The serum was treated with 2-mercaptoethanol (2-ME) to determine the immunoglobulin class and titer of the antibody. Results The irregular antibody screen was positive, and the antibody was specifically identified as anti-K. It was characterized as a mixed IgM and IgG isotypes, with an IgM titer of 2 and an IgG titer of 256. Conclusion Utilization of suitable laboratory techniques allows for the accurate detection of IgM+IgG anti-K antibodies, providing a guarantee for safe clinical transfusion.

Key words: Kell blood group, Anti-K antibody, Antibody identification, IgM antibody, IgG antibody

中图分类号:

R457.1⁺1

康世蓉, 王丹, 袁小华, 陆红芹, 李苏亮. 一例IgM+IgG型抗-K抗体鉴定与分析[J]. 临床输血与检验, 2026, 28(3): 425-429.

KANG Shirong, WANG Dan, YUAN Xiaohua, LU Hongqin, LI Suliang. A Case of Mixed IgM and IgG Anti-K Antibody: Identification and Clinical Implications[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(3): 425-429.

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一例IgM+IgG型抗-K抗体鉴定与分析

A Case of Mixed IgM and IgG Anti-K Antibody: Identification and Clinical Implications

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