• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2026, Vol. 28 ›› Issue (1): 36-43.DOI: 10.3969/j.issn.1671-2587.2026.01.006

• 基础研究 • 上一篇    下一篇

三种光源的亚甲蓝光化学法血浆病毒灭活效果比较*

莫琴, 黄宇闻, 邱颖婕, 刘鸿, 伍晓菲, 贾尧, 马荣钠, 王迅   

  1. 上海市血液中心，上海 200051
  • 收稿日期:2025-05-29 发布日期:2026-02-13
  • 通讯作者: 王迅，主要从事输血传染病、血液筛查质量评估研究，（E-mail）wangxun@sbc.org.cn。
  • 作者简介:莫琴，主要从事血液制品病原体灭活研究，（E-mail）moqin@sbc.org.cn。
  • 基金资助:
    *本课题受上海市卫生健康委员会卫生行业临床研究专项面上项目（No.202240210）资助

Comparison of Plasma Viral Inactivation Efficacy of Methylene Blue Plus Light Treatment Using Three Light Sources

MO Qin, HUANG Yuwen, QIU Yingjie, LIU Hong, WU Xiaofei, JIA Yao, MA Rongna, WANG Xun   

  1. Shanghai Blood Center, Shanghai 200051
  • Received:2025-05-29 Published:2026-02-13

PDF

5

摘要： 目的 比较白光LED、红光LED以及荧光灯为光源的亚甲蓝光化学法对血浆病毒灭活效果。方法 血浆中加入终浓度为1 μmol/L的亚甲蓝，分别用白光LED、红光LED和荧光灯照射30 min，比较处理后血浆中凝血因子（纤维蛋白原[FIB]、因子[F]Ⅱ、FⅤ、FⅦ、FⅧ、FⅨ、FⅩ、FⅪ、FⅫ、血管性假血友病因子抗原[vWF:Ag]、血管性假血友病因子活性[vWF:Ac]）、抗凝血蛋白（抗凝血酶[AT]、蛋白C[PC]）的活性、凝血酶原时间（PT）和部分活化凝血酶原时间（APTT），以及对血浆中Sindbis病毒的灭活效果。结果 三种光源的亚甲蓝光化学法处理都会使血浆中凝血因子和抗凝血蛋白活性受损，凝血时间延长，其中FⅤ、FⅦ、FⅨ和FⅪ、vWF:Ac和AT的活性变化在三种光源间没有显著性差异（P=0.115 8，0.434 2，0.610 8，0.094 3，0.137 7，0.166 7），PT、APTT的凝血时间变化也没有显著性差异（P=0.652 6，0.138 2）。红光LED的FIB、FⅧ、FⅫ和PC低于白光LED（P<0.000 1，P<0.000 1，P=0.023 2，P<0.000 1）和荧光灯（P<0.000 1，P<0.000 1，P=0.003 4，P<0.000 1）。荧光灯的FⅡ高于红光LED（P=0.012 2），FⅩ高于白光LED和红光LED（P=0.036 6，0.006 8）。白光LED的vWF:Ag显著高于红光LED和荧光灯（P=0.014 3，0.017 2）。经过三种光源的亚甲蓝光化学处理，血浆中的Sindbis病毒效价下降均>4 LogTCID50/0.1 mL。结论 三种光源的亚甲蓝光化学法处理都能有效灭活血浆中的Sindbis，三种光源中红光LED对血浆中FIB和FⅧ活性影响最大，白光LED可作为未来血浆病毒灭活处理的首选光源。

关键词: 不同光源LED, 亚甲蓝光化学法, 凝血因子, 血浆病毒灭活

Abstract: Objective To compare the viral inactivation effects of methylene blue(MB) plus light (MB/light) treatment using white light LEDs, red light LEDs, and fluorescent lamps on plasma. Methods Plasma samples were treated with methylene blue at a final concentration of 1 μM, followed by 30 minutes of irradiation using white light LEDs, red light LEDs, or fluorescent lamps. The study evaluated the impact of these treatments on coagulation factors (FIB, FⅡ, FⅤ, FⅦ, FⅧ、FⅨ, FⅩ, FⅪ, FⅫ, vWF:Ag, vWF:Ac), anticoagulant proteins (AT, PC), PT, and APTT. Additionally, the efficacy of SINDBIS virus inactivation in plasma was assessed. Results All three methylene blue photodynamic therapies significantly reduced the activity of coagulation factors and anticoagulant proteins, and prolonged coagulation times. No significant differences were found in the activity of FⅤ, FⅦ, FⅨ, FⅪ, vWF:Ac, and AT among the three light sources (P=0.115 8, 0.434 2, 0.610 8, 0.094 3, 0.137 7, 0.166 7). There were no significant differences in the changes of PT and APTT (P=0.652 6, 0.138 2). Red light LEDs treatment resulted in lower FIB, FⅧ, FⅫ, and PC levels compared to white light LED (P<0.000 1, P<0.000 1, P=0.023 2, P<0.000 1) and fluorescent lamps (P<0.000 1, P<0.000 1, P=0.003 4, P<0.000 1). Fluorescent lamps treatment resulted in higher FII levels than red light LED (P=0.012 2), and higher FX levels than both white light LEDs and red light LEDs (P=0.036 6, 0.006 8). White light LEDs treatment yielded higher vWF:Ag levels than red light LED and fluorescent lamps (P=0.014 3, 0.017 2). Following MB/light treatment with the three light sources, the Sindbis virus titer in plasma decreased by>4 LogTCID50/0.1 mL. Conclusion All three MB/light treatments effectively inactivated Sindbis virus in plasma. Red light LED had the most significant effect on FIB and FⅧ activity. White light LED is the preferred light source for future plasma viral inactivation treatments.

Key words: Different light source LEDs, Methylene blue photochemical method, Coagulation factor, Plasma viral inactivation

中图分类号: