NFKBIZ基因在乳腺癌中的生物学功能及临床诊断价值研究*

doi:10.3969/j.issn.1671-2587.2022.04.014

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (4): 482-487.DOI: 10.3969/j.issn.1671-2587.2022.04.014

NFKBIZ基因在乳腺癌中的生物学功能及临床诊断价值研究^*

吴军营, 王凌霞, 吴茜茜, 朱志宸, 王波, 杨欢

215004 苏州大学附属第二医院检验科（吴军营,王凌霞,吴茜茜,朱志宸,杨欢）; 肿瘤科（王波）; 输血科（杨欢）; 苏州大学附属儿童医院检验科（吴军营）; 放射医学与辐射防护国家重点实验室（苏州大学）（杨欢）

收稿日期:2022-02-07 出版日期:2022-08-20 发布日期:2022-08-19
通讯作者: 杨欢,主任技师,副教授,主要从事输血及肿瘤学研究,（E-mail）zjyhcherry@126.com。
作者简介:吴军营（1987-）,男,江苏盐城人,主管技师,在读硕士,主要从事凝血相关检验工作,（E-mail）450337203@qq.com。
基金资助:
*本课题受国家自然科学基金青年项目（No.81702078）、江苏省自然科学基金（No.BK20170356）、苏州市科技计划项目（No.SKY2021045、SKJY2021097）、江苏省妇幼保健协会项目（No.FYX202123）、“姑苏卫生人才计划”青年拔尖人才项目、核技术医学应用重点人才项目（No. XKTJ-HRC2021001）、放射医学与辐射防护国家重点实验室资助项目（No.GZK1202137）和苏州市“科教兴卫”青年科技项目（No.KJXW2020019）资助

NFKBIZ in Breast Cancer：Biological Function and Clinical Diagnostic Value

WU Jun-ying, WANG Ling-xia, WU Xi-xi, et al

Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004

Received:2022-02-07 Online:2022-08-20 Published:2022-08-19

摘要/Abstract

摘要： 目的研究NFKBIZ基因在乳腺癌中的生物学功能并结合临床资料分析其临床意义,探讨其作为诊断标志物的潜在价值。方法利用实时荧光定量PCR（qPCR）技术对乳腺癌细胞系、组织中的NFKBIZ基因进行检测;利用蛋白质免疫印迹（western blot）、免疫组化对乳腺癌细胞系、组织中的IκBζ蛋白进行检测;构建siRNA并转染乳腺癌细胞后进行平板克隆实验和迁移实验,检测NFKBIZ基因的表达对乳腺癌细胞增殖及迁移能力的影响;通过绘制ROC曲线分析NFKBIZ基因在乳腺癌中的诊断效能;用统计学方法分析NFKBIZ基因表达水平与乳腺癌病理参数的相关性。结果 qPCR结果显示,MDA-MB-231中表达水平明显高于MCF-7（P<0.05）;与癌旁组织相比,乳腺癌组织内NFKBIZ基因表达水平明显上调,差异具有统计学意义（P<0.05）;乳腺癌中IκBζ蛋白的表达水平明显高于良性乳腺病组织;NFKBIZ基因的ROC曲线下面积（AUC）是0.810,cutoff值为1.418,敏感性为81.8%,特异性为90.9%。NFKBIZ基因表达水平与肿瘤大小和患者年龄存在显著相关性（P<0.05）,而与TNM分期、病理类型、淋巴结转移无显著相关性（P>0.05）。结论 NFKBIZ基因在乳腺癌发展过程中发挥重要作用,并可能成为临床诊断的新指标。

关键词: 乳腺癌, NFKBIZ基因, IκBζ, ROC, 诊断价值

Abstract: Objective We studied the biological function of NFKBIZ in breast cancer and analyzed its clinical significance in combination with clinical data to discuss its potential value of diagnosis. Methods Quantitative real-time PCR（qPCR） was used to identify the expression of NFKBIZ in cells and in tissues. Western blot assay and immunohistochemistry were used to detect the IκBζ expression in tissues. siRNA was constructed and transfected into breast cancer cells for colony formation and transwell migration assay to detect the effect of NFKBIZ expression on the proliferation and migration; ROC curve was performed to analyze the diagnostic efficacy of NFKBIZ in breast cancer; Statistical analysis was determined the correlation between NFKBIZ expression level and pathological parameters of breast cancer.Results qPCR showed that the expression of NFKBIZ on MDA-MB-231 cells was significantly higher than that on MCF-7 cells （P<0.05）,and its expression in breast cancer tissues was significantly higher than that in para-cancerous tissues（P<0.05）. The expression level of IκBζ protein in breast cancer was significantly higher than that in benign breast disease tissues. The area under the ROC curve （AUC） of NFKBIZ was 0.810, with a cutoff value of 1.418,showing a sensitivity of 81.8%, and a specificity of 90.9%. The expression level of NFKBIZ was significantly correlated with tumor size and patient age （P<0.05）,but it had no significant correlation with TNM stage,pathological type,and lymph node metastasis （P>0.05）.Conclusion NFKBIZ plays an important role in the development of breast cancer and may become a novel biomarker for clinical diagnosis.

Key words: Breast cancer, NFKBIZ, IκBζ, ROC, Diagnostic value

中图分类号:

R737.9

吴军营, 王凌霞, 吴茜茜, 朱志宸, 王波, 杨欢. NFKBIZ基因在乳腺癌中的生物学功能及临床诊断价值研究^*[J]. 临床输血与检验, 2022, 24(4): 482-487.

WU Jun-ying, WANG Ling-xia, WU Xi-xi, et al. NFKBIZ in Breast Cancer：Biological Function and Clinical Diagnostic Value[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(4): 482-487.

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NFKBIZ基因在乳腺癌中的生物学功能及临床诊断价值研究^*

NFKBIZ in Breast Cancer：Biological Function and Clinical Diagnostic Value

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