• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2019, Vol. 21 ›› Issue (4): 387-389.DOI: 10.3969/j.issn.1671-2587.2019.04.014

• 血液检测 • 上一篇    下一篇

混样核酸检测HBV拆分阴性标本的血清学标志物分析*

蒋昵真, 王金花, 王亚武, 冯雅颂, 黄成垠, 赵红   

  1. 210042 南京,江苏省血液中心 （蒋昵真,王金花,王亚武,冯雅颂,黄成垠）; 南京市第二医院（赵红）
  • 收稿日期:2018-04-03 出版日期:2019-08-20 发布日期:2019-08-28
  • 通讯作者: 黄成垠,男,主要从事输血研究及管理工作,（E-mail）huangchengyin1223@126.com。
  • 作者简介:蒋昵真（1981-）,女,江苏常州人,助理研究员,硕士,主要从事输血与检验工作,（E-mail）jiangnizhen@hotmail.com。
  • 基金资助:
    *本课题受江苏省“333高层次人才培养工程”（No.2016-Ⅲ-3345）,南京市卫计委重点项目（No.ZKX15044）资助

An Analysis of HBV Serological Markers in the Samples of Positive Minipool NAT with Negative Resolution NAT

JIANG Ni-zhen, WANG Jin-hua, WANG Ya-wu, et al   

  1. Jiangsu Provincial Blood Center,Nanjing 210042
  • Received:2018-04-03 Online:2019-08-20 Published:2019-08-28

PDF

1102

摘要： 目的 探讨混样核酸检测HBV拆分阴性的献血者标本是否存在低浓度HBV DNA。方法 检测并比较混样核酸检测HBV拆分阴性标本与正常标本、HBV DNA拆分（+）/HBV DNA定量（-）三种标本的HBV血清学标志物（HBsAg、抗-HBs、抗-HBc、HBeAg、抗-HBe）的电化学发光检测结果;对HBV拆分阴性者增加1遍拆分。结果 ①693例混样核酸检测HBV拆分阴性标本中：单独抗-HBs 239例,抗-HBs/抗-HBc 134例,抗-HBs/抗-HBe/抗-HBc 111例,单独抗-HBc 25例,抗-HBe/抗-HBc 9例,HBV血清学标志物阳性率74.75%（518/693）,虽与正常标本阳性率（71.00%）的差异无统计学意义,但抗-HBs和抗-HBs/抗-HBc模式的占比与正常标本存在差异。②275例HBV DNA拆分（+）标本中有119例HBV DNA定量检测为（-）,其中抗-HBe/抗-HBc 38例,抗-HBs/抗-HBc 30例,抗-HBs/抗-HBe/抗-HBc 22例,单独抗-HBc 18例,单独抗-HBs 3例,HBsAg/抗-HBe/抗-HBc 4例,其HBV血清学标志物阳性率为96.64%（115/119）,与混样核酸检测HBV拆分阴性标本的差异有统计学意义,但抗-HBs/抗-HBc和抗-HBs/抗-HBe/抗-HBc模式的占比与混样核酸检测HBV拆分阴性标本的差异无统计学意义。③29次2次拆分中有6次可以检出HBV DNA。结论 当混样核酸检测HBV拆分阴性标本存在抗HBs伴抗HBc时,可能有HBV感染风险。

关键词: 拆分阴性, 乙型肝炎病毒, 血清学标志物

Abstract: Objective To investigate blood donor samples of positive minipool-HBV NAT （MP-HBV）but negative resolution HBV NAT （resolution-HBV）whether contain trace HBV DNA. Methods Samples of positive MP-HBV but negative resolution-HBV,and positive resolution-HBV but negative quantitative HBV,and normal samples,were measured by electrochemiluminescence assay （ECLIA）for HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc. The resolution NAT was executed once more when resolution NAT was negative. Results Among 693 samples of positive MP-HBV with negative resolution-HBV,239 showed only anti-HBs,134 demonstrated anti-HBs/anti-HBc,111 with anti-HBs/anti-HBe/anti-HBc,25 with only anti-HBc and 9 with anti-HBe/anti-HBc. No significant difference of the seroprevalence was noted between these samples （74.75%）and normal samples （71.00%）. There were 119 of 275 resolution-HBV positive samples that presented quantitatively HBV negative. Among them,38 showed positive anti-HBe/anti-HBc,30 were anti-HBs/anti-HBc,22 anti-HBs/anti-HBe/anti-HBc,18 were only anti-HBc,3 only anti-HBs and 4 were HBsAg/ anti-HBe/anti-HBc. The HBV seroprevalence was 96.64%,which was higher than positive MP-HBV but negative resolution-HBV samples. Six of 29 secondary resolution NAT revealed positive HBV DNA. Conclusion There may exist HBV infection in positive MP-HBV but negative resolution-HBV samples with anti-HBs/anti-HBc.

Key words: Negative, Resolution NAT HBV, Serological markers

中图分类号: