Effect of Freeze-dried Platelet-rich Plasma on the Proliferation and Migration of Human Dermal Fibroblasts in Vitro

doi:10.3969/j.issn.1671-2587.2026.03.010

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2026, Vol. 28 ›› Issue (3): 359-362.DOI: 10.3969/j.issn.1671-2587.2026.03.010

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Effect of Freeze-dried Platelet-rich Plasma on the Proliferation and Migration of Human Dermal Fibroblasts in Vitro

LI Yanhui, PI Liping, XIE Tingting, ZHOU Mou, SHI Linying

Blood Transfusion, General Hospital of Southern Theatre Command, Guangzhou, Guangdong 510010

Received:2025-08-22 Published:2026-07-07

Abstract

Abstract: Objective To evaluate the capacity of freeze-dried platelet-rich plasma (FD-PRP) to stimulate the proliferation and migration of human dermal fibroblasts. Methods Freshly isolated PRP was lyophilized to generate FD-PRP. Cell proliferation was quantified using the CCK-8 assay; directional migration was monitored via scratch-wound assays; and the secretion of type Ⅰ collagen and elastin was measured by ELISA. Results Compared to untreated controls, FD-PRP significantly enhanced fibroblast proliferation and increased the secretion of type Ⅰ collagen and elastin (P<0.05). These effects were statistically comparable to those induced by fresh PRP (P>0.05). Scratch-wound assays further revealed that both FD-PRP and fresh PRP markedly enhanced cell migration into the denuded area compared with the controls. Conclusion FD-PRP induces the proliferation and migration of adult dermal fibroblasts and also enhances the secretion of type Ⅰ collagen and elastin. These findings lay a solid experimental foundation for the potential clinical application of FD-PRP in the future treatment of various wound types.

Key words: Platelet rich plasma, Freeze-dried, Human fibroblasts, Wound

CLC Number:

R331.1⁺43R457.1⁺4

LI Yanhui, PI Liping, XIE Tingting, ZHOU Mou, SHI Linying. Effect of Freeze-dried Platelet-rich Plasma on the Proliferation and Migration of Human Dermal Fibroblasts in Vitro[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(3): 359-362.

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Effect of Freeze-dried Platelet-rich Plasma on the Proliferation and Migration of Human Dermal Fibroblasts in Vitro

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