Abstract: Objective To explore the enhancement effect of polyethylene glycol microcolumn gel method （PEG-MGT） in irregular antibody detection. Methods We retrospectively analyzed 70 specimens with weak agglutination by low ionic strength salt solution-microcolumn gel method（LISS-MGT） as well as additional PEG-MGT results from April 2018 to April 2021 and compared the 16-pannel cell reaction pattern and experimental results. Results We identified the specific antibodies in 29 cases by PEG-MGT（41.4%）,including 20 cases of Rh system-related antibodies（69%）,5 cases of Duffy system antibodies （17.2%）,3 cases of Kidd system antibodies （10.3%） and one case of Lewis system antibodies（3.4% ）. There were still 41 cases（58.6%）to be determined the specificity of antibodies. By comparing with the reaction intensity of LISS -MGT and the PEG-MGT results,the experimental results showed that PEG-MGT enhanced the reaction intensity in 16 cases,weakened in 17 cases,and unchanged in 8 cases. The antibodies could be determined by saline medium or MGT incubating at 4℃ in a total of 13 cases,including 9 cases of anti-M antibodies（4 cases of PEG-MGT enhancement,3 cases of weakening,4 cases of constant strength）,2 cases of anti-P1 antibody（both PEG-MGT enhancement）,and anti-Lea antibody （PEG-MGT enhancement） and anti-Leb antibody （PEG-MGT weakening） one case each. Conclusion PEG-MGT method could significantly enhance the antigen-antibody response in Rh system, Duffy system and Kidd system, and it also could be helpful to determine antibodies of MNS system, Lewis system and P1 system.

Key words: PEG, LISS, MGT, Antibody, identification