Abstract: Objective To explore the effects of resveratrol on platelet oxidative stress and aging index levels and storage time. Methods Plasma rich in platelets was prepared from 6-week-old C57 black 6 mice. Each sample was equally divided into observation group and control group. The observation group was added with resveratrol, and dimethyl sulfoxide was used to calibrate it to 50 μmmol /L, the control group was added with the same amount of dimethyl sulfoxide. Stored in 1, 2, 3, 4, 5, 6, and 7 days for sampling, testing content including: mean platelet volume（MPV）, platelet count, pH, glucose levels, reactive oxygen species（ROS）level, proportion of Senescence-associatedβ-galactosidase staining positive cells, platelet CD62P, membrane surface proteins associated protein Ⅴ and MitoSpy positive level. Results During the observation period, there was no significant difference in MPV, platelet count, pH, and glucose level between the two groups at each time point（P>0.05）. The ROS level of the observation group at the 5th and 6th day of storage were significantly lower than control group（t=9.977,10.650, P=0.000, 0.000）, the SA-β-gal activity intensity of the observation group at the 2 d, 3 d, 4 d, 5 d, 6 d, 7 d were significantly higher than control group（t=3.237, 4.004, 4.909, 3.875, 4.392, 3.359; P=0.013, 0.001, 0.000, 0.002, 0.000, 0.010）. There was no statistical difference between the platelet spreading and aggregation levels observed at 1d（P>0.05）, the spreading and aggregation levels of the observation group at 3 d and 5 d were significantly above（t=3.096, 3.396, P=0.015, 0.007）. Observation group in the storage of 2 d, 3d, 4 d, 5 d, 6 d, 7 d days of platelet CD62P activity were significantly lower than the control group（t=3.175, 3.345, 3.851, 5.169, 4.358, 7.601, P=0.017, 0.010, 0.002, 0.000, 0.000, 0.000）, the storage of 3 d, 4 d, 5 d, 6 d, 7 d days of membrane protein V activated platelets were significantly lower than the control group（t=13.480,13.260,14.870,3.223,8.716;P=0.000,0.000,0.000,0.015,0.000）, the storage of 5 d,6 d, 7 d MitoSpy positive blood platelet were significantly higher than control group（t=4.279,7.702,10.270;P=0.000,0.000,0.000）. Conclusion sResveratrol can significantly improve platelet oxidative stress, aging index,the spreading and aggregation and the storage time during storage, it has good application potential.

Key words: Resveratrol, Platelets, Oxidative stress, Aging