Abstract: Objective Analysis of pertussis infection in Shandong area. Methods From January 2015 to December 2016 in Shangdong Provincial Hospital affiliated to Shandong University,BP-DNA analysis was used to detect Bordetella pertussis nucleic acid in 1989 patients. SPSS21.0 software was used to retrospectively analyse Bordetella pertussis infection in population. Results In 1989 throat swab specimens,the positive rate of nucleic acid detection of Bordetella pertussis was 23.06%,the positive rate of pertussis nucleic acid is 30.78% in male,slightly lower than the 34.75% of women,but there was not statistically significant difference between two groups （P>0.05）; the positive rate of nucleic acid of Bordetella pertussis in the age group of 0-1 year old is the highest,accounting for 38.72%,the positive rate decreased gradually with the age increasing,and the positive rate is the lowest in above 30 years old group with 14%; there was significant difference between seasons of Bordetella pertussis nucleic acid detection positive rate （P<0.05）. Among them,the highest positive rate was 40.35% in summer,which was significantly higher than that in autumn with 33.11% （P<0.05）,the positive rate in autumn was significantly higher than that in spring with 24.8% （P<0.05）,and it was slightly higher in spring than in winter with 19.56% （P>0.05）. Conclusions There was no significant difference in the positive rate of detection of Bordetella pertussis among population in Shandong area,but there were differences in age and season. We should strengthen the detection of Bordetella pertussis nucleic acid in suspected patients.

Key words: Bordetella pertussis, Nucleic acid, Shandong