• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

临床输血与检验 ›› 2019, Vol. 21 ›› Issue (5): 491-492.DOI: 10.3969/j.issn.1671-2587.2019.05.013

• 血液检测 • 上一篇    下一篇

54例HIV抗体确认阳性无偿献血者蛋白印迹实验带型分析

陈秀莲, 韩雪莹, 李华   

  1. 250001 山东省济南市血液供保中心
  • 收稿日期:2018-07-03 出版日期:2019-10-20 发布日期:2019-10-29
  • 通讯作者: 韩雪莹,女,主任技师,主要从事采供血血液检测工作,(E-mail)63588784@qq.com。
  • 作者简介:陈秀莲(1980-),女,山东莱芜人,主管技师,主要从事采供血血液检测工作,(Tel)18553119589。

Analysis of Band Patterns of Western Blot Test in 54 HIV Antibody Positive Blood Donors

CHEN Xiu-lian, HAN Xue-ying, LI Hua   

  1. Jinan Blood Supply and Security Center,Jinan 250001
  • Received:2018-07-03 Online:2019-10-20 Published:2019-10-29

摘要: 目的 对本地区HIV抗体确认阳性献血者的带型特征进行分析,以对献血招募、职业防护及血液检测提供理论指导。方法 经本中心实验室初筛检测为HIV抗体反应性标本送市疾控中心进行蛋白免疫印迹法(western blotting,WB)确认检测,收集经确认实验为HIV抗体阳性的标本信息,对各条带出现率及条带组合情况分别进行汇总分析。结果 共收集HIV抗体确认阳性献血者54例,带型以全带型比例最高,为38.9%;各条带检出率:gp160/120、p24均为100%,其次为p66、gp41分别为92.6%、90.7%,p31、p17、p51依次为83.3%、87.0%、87.0%,p39和p55阳性率最低,分别为59.2%、42.6%。结论 带型分析显示HIV抗体确认阳性献血者以早期感染为主,应采取措施避免高危人群献血。

关键词: HIV, 带型, 献血者, 蛋白免疫印迹确认实验

Abstract: Objective To provide theoretical support for recruitment of blood donors,occupational protection of our staffers as well as blood test we did study on the band patterns of HIV antibody positive.Methods Whole blood samples of the blood donors’were tested by ELISA screening test in our laboratory and those HIV antibody reactive samples were sent to Ji Nan CDC for confirmatory test using Western blotting technology. Collect the positive information feedback and take a pooled analysis of band patterns by analyzing the rate of the band appearance as well as band patterns.Results Totally 54 positive cases were collected. Reactivity with all the bands was the most commonly observed WB pattern,which occurred 38.9%in HIV antibody positive blood donors. The bands of gp160/120 and p24 were observed in all the patients. The next is p66 (92.6%) and gp41(90.7%). The positive rate of p31,p17,p51 were 83.3%,87.0%,87.0% respectively. The bands of p39 and p55 showed low positive rates (59.2% and 42.6%,respectively).Conclusion The band patterns indicate that HIV infection is in the early stage in most of the HIV antibody positive blood donors. Measures should be taken to avoid blood donation from those high risk population.

Key words: HIV, Band pattern, Blood donors, Western blotting

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