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临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (6): 602-605.DOI: 10.3969/j.issn.1671-2587.2017.06.021

• 临床检验 • 上一篇    下一篇

抗-HCV-IgG 抗体及血清HCV RNA在丙型肝炎诊断中的应用价值*

王伟, 葛亮, 居朝霞   

  1. 210028 江苏省疾病预防控制中心检验科（王伟）;
    江苏省中西医结合医院检验科（葛亮）;
    江苏省无锡市第五人民医院传染科（居朝霞）
  • 收稿日期:2017-06-12 出版日期:2017-12-20 发布日期:2017-12-13
  • 作者简介:王伟（1986-）,女,江苏南京人,主管技师,学士,主要从事免疫学工作,（E-mail）wer09w@163.com。
  • 基金资助:
    *本课题受无锡市医院管理中心科研项目（No; YGZXH14010）资助

Anti-HCV-RNA Antibody and Serum HCV-RNA in the Diagnosis of Hepatitis C

WANG Wei, GE Liang, JU Zhao-xia   

  1. Department of Clinical Laboratory, Jiangsu Provincial Lenter for Disease Control and Prevention, nanjing Jiangsu, 210028
  • Received:2017-06-12 Online:2017-12-20 Published:2017-12-13

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摘要： 目的 探讨抗-HCV-IgG抗体与血清HCV RNA应用于丙型肝炎诊断中的临床价值。方法 选择2014年10月~2016年10月检验科收集的114例丙型肝炎待查者的血清标本,采用酶联免疫吸附法（ELISA）对抗-HCV-IgG抗体进行检测,同时采用荧光定量聚合酶链反应（PCR）对HCV RNA载量进行检测。统计所有标本抗-HCV-IgG抗体、HCV RNA检测结果。结果 采用ELISA检测抗-HCV-IgG抗体阳性率为25.44%（29/114）;采用荧光定量PCR检测HCV RNA阳性率为27.19%（31/114）。31例HCV RNA阳性标本中抗-HCV-IgG阳性标本有26例,符合率为83.87%（26/31）。ELISA检测抗-HCV-IgG抗体阳性率与荧光定量PCR检测HCV RNA阳性率的差异无统计学意义（P>0.05）。伴随HCV RNA病毒载量的不断增多,抗-HCV-IgG阳性抗体检出率明显提高;不同HCV RNA病毒载量的相邻区间的抗-HCV-IgG阳性抗体检出率比较,差异无统计学意义（P>0.05）。结论 ELISA检测抗-HCV-IgG抗体和荧光定量PCR检测HCV RNA应用于丙型肝炎诊断均具有一定的临床价值,血清HCV RNA病毒载量是判断HCV感染的重要依据,能够有效反映病毒活动性及复制程度,然而单一的抗-HCV-IgG抗体、HCV RNA检测尚存在局限性,易出现漏诊,两者联合检测可显著提高丙型肝炎检出率,做到早诊断、早治疗,提高临床疗效。

关键词: 抗-HCV-IgG抗体, HCV RNA, 丙型肝炎, 诊断, 临床价值

Abstract: Objective To investigate the clinical value of anti-HCV-IgG antibody and serum HCV-RNA in the diagnosis of hepatitis C. Methods The serum samples of 114 patients with hepatitis C were collected from October 2014 to October 2016,the anti-HCV-RNA antibody was detected by enzyme-linked immunosorbent assay （ELISA）,and the HCV-RNA load was detected by fluorescence quantitative polymerase chain reaction （PCR）.Statistics of all specimens of anti-HCV-RNA antibody,HCV-RNA data. Results The positive rate of anti-HCV-IgG was 25.44% （29/114）by ELISA. The positive rate of HCV-RNA was 27.19% （31/114）by fluorescence quantitative PCR. anti-HCV-IgG positive specimens of 26 cases were detected from 31 cases of HCV-RNA positive specimens,the coincidence rate of 83.87% （26/31）. There was no significant difference in detection positive rate between anti-HCV-IgG antibody by ELISA and HCV-RNA by fluorescence quantitative PCR （P> 0.05）. With the increase of HCV-RNA viral load,the detection rate of anti-HCV-IgG positive antibody was significantly increased. There was no significant difference in the detection rate of anti-HCV-IgG antibody positive in the adjacent region of different HCV-RNA viral load （P> 0.05）.Conclusion ELISA to detect anti-HCV-RNA antibody and fluorescence quantitative PCR detection of HCV-RNA in the diagnosis of hepatitis C have a certain clinical value,serum HCV-RNA viral load is an important basis for judging the infection of hepatitis C virus,which can But a single anti-HCV-RNA antibody,HCV-RNA detection is still limited,prone to misdiagnosis phenomenon,the combination of the two can significantly improve the detection rate of hepatitis C virus infection,Do early diagnosis,early treatment,to provide value for improving clinical efficacy.

Key words: Anti-HCV-IgG antibody, HCV-RNA, Hepatitis C, Diagnosis, Clinical value

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