Abstract: Objective To detect respiratory pathogens in patients with acute exacerbation underlying chronic obstructive pulmonary disease （AECOPD） so as to provide evidences for clinical treatments.Methods A retrospective analysis was performed in the hospital from May 2015 to April 2016. 478 cases of AECOPD were examined for IgM antibodies by IFA,and pathogens by sputum culture for influenza virus A/B,para influenza virus,respiratory syncytial virus,adenovirus and atypical pathogens （Legionella pneumophila,Mycoplasma pneumonia,and Chlamydia pneumonia）.Results The overall positive rate of pathogens was 78.0% （373/478）,single species infection accounted for 41.2% （197/478）,viral infection accounted for 58.4%,and multiple pathogen infections accounted for 58.7% （219/373）. Among them,influenza virus B,M. pneumonia and L. pneumophilia,influenza virus A and Candida infection dominates both in total prevalence and single species pathogen infection. Males had a higher positive rate of 54.7% （204/478） than females （45.3%,169/478） （χ²=4.06,P=0.044） Virus and atypical pathogen infections varied in gender （χ²=4.7,P=0.03,χ²=19.83,P=0）; fungus infection rate changedwith seasons （χ²=11.85,P=0.008）.Conclusion sThe causative agents in AE-COPD mainly are comprised of viruses and atypical pathogens,influenza virus B,M. pneumonia,influenza virus A,L. pneumophila and Candida,single species or mixed infections are common.

Key words: Acute exacerbation of chronic obstructive pulmonary disease, Respiratory, Infection, Pathogen