Abstract: Objective To investigate the effect of Qingkailing injections on in vitro model of massive blood loss. Methods Eight apheresis platelets (≥250 mL / sample) collected were equally divided into 2×125 mL. A final concentration of 1% Qingkailing injections (1.25 mL in 125 mL) as the experimental group was stored at 22℃ with constant shaking. The control group did not add Qingkailing injections. The model of massive blood loss in vitro was established by blood dilution method (whole blood:saline =1:9). The thrombelastography（TEG）indexes and blood routine indexes were compared to discuss the correction effect of leukocyte-reduced red blood cells (RBCs), fresh frozen plasma (FFP) and platelets with or without Qingkailing injections in a 1:1:1 ratio on in vitro model of massive blood loss. Results After platelets were stored for 1, 3, and 5 days, Platelets (×10⁹/L) in the experimental group and the control group increased from 19~20 to 68~72 vs. 57~64 (P<0.05), respectively. The TEG-MA value (mm) increased from 9.6~11.4 to 49.1~58.3 vs. 48.1~55.1 (P<0.05), however the TEG-R value (min) decreased from 7.1~8.2 to 3.9~4.6 vs. 3.7~4.0 (P>0.05). After 7~14 days, platelets (×10⁹/L) were corrected from 18 to 56~62, TEG-MA value (mm) from 10.4~12.1 to 43.3~47.9, and TEG-R value (min) was corrected from 8.2~8.9 to 3.6~5.5 (P<0.05). Conclusion In vitro model of massive blood loss could be corrected by leukocyte-reduced red blood cells, fresh frozen plasma and platelets preserved with Qingkailing injections, suggesting that platelets stored for 10 days with Qingkailing injections had a correction effect.

Key words: Qingkailing injections, Platelet, Massive blood loss model, Correction of blood loss, Hemostasis