Abstract: Objective To investigate the distribution characteristics of HLA-A and -B loci and HPA-1~6w, 10w, 15, and 21w polymorphisms in regular platelet donors in Nanjing area, and to evaluate the minimum capacity of the regular platelet donor gene database. Methods Fluorescence quantitative PCR was used to genotype for HLA A, -B loci and HPA-1~6w, 10w, 15, 21w genes in blood samples of 1 059 platelet regular donors in Nanjing area, and the allele frequency, HLA haplotype frequency and HPA combination frequency were calculated, and based on these data, the storage capacity level was evaluated. Results Among the 1 059 regular platelet donors, a total of 15 alleles in HLA-A loci were detected, of which A*02, A*11, A*24, A*33, A*30 had higher frequencies; 29 alleles in HLA-B loci were detected, of which B*15, B*40, B*13, B*46, B*51, B*58 had higher allele frequencies; 269 HLA-A-B haplotypes were found, and 22 had frequencies >0.01, of which A*02-B*15 and A*30-B*13 were the top two highest frequencies. Among the HPA-1~6w, 10w, 15, and 21w , the HPA-1, 2, 4, 5, 6w, and 21w were dominated by aa genotypes, and bb was not detected in HPA-1, 4, 5, 6w, and 21 w; HPA-3 and HPA-15 have high heterozygosity; a total of 59 HPA combinations were detected, and the highest one was 1aa-2aa-3ab-4aa-5aa-6aa-10aa-15ab-21aa. In the genetic database of 1 059 donors established in this region, 88.97% of the patients could search ≥1 HLA-A, B matched donor in this database. It was deduced by calculation that when the number of platelet donors was 213, 95% of the patients could search at least one HPA-1~6w, 10w, 15, 21w compatible donor. Conclusion The distribution of HLA and HPA genotypes of platelet regular donors in Nanjing area has unique polymorphism in this region. The gene database of 1059 individuals established in this study can basically meet the need of seeking HLA and HPA compatible donors for patients with ineffective platelet transfusion in this region.

Key words: Platelet, HLA, HPA, Gene polymorphism, Capacity assessment