Abstract: Objective To understand the genetic polymorphism of HLA-A,B alleles and HPA1-17 in platelet donors in Zibo area, and establish a database of platelet donors with HLA and HPA locus. Methods Polymerase chain reaction-sequencing based typing (PCR-SBT) and Polymerase chain reaction-sequence specific primer (PCR-SSP) methods were applied in genotyping for HLA-A,B and HPA1-17, respectively.Total of 200 blood samples were collected from unrelated platelet donors．The gene frequencies of HLA-A、B and HPA1-17 were calculated using direct counting method. Results The numbers of low-resolution alleles identified were 15 for HLA-A and 29 for HLA-B,respectively. The most frequent alleles at HLA-A locus were A^*02、A^*24、A^*11、A^*30、A^*33,with a gene frequency in 0.260 0、0.172 5、0.137 5、0.135 0、0.070 0,respectively．For HLA-B locus,the common alleles were B^*13、B^*15、B^*40、B^*44、B^*51,with a gene frequency of 0.160 0、0.132 5、0.130 0、0.067 5、0.062 5,respectively.Within HPA4a, 7a-14a, 16a and 17a systems, only HPA-aa genotype were found. Findings of HPA-15 showed the greatest heterozygosity with a genotype frequency of 0.275 0、0.530 0 and 0.195 0 for HPA15a/15a,HPA15a/15b,and HPA15b /15b,respectively. Conclusion The distribution of HLA-A,B and HPA1-17 in platelet donors in Zibo area have been explored. This research can contribute to establish a regional bank of platelet donors in Shandong province.

Key words: HLA, HPA, Gene frequency, Bank of platelet donors