• 中国科学论文统计源期刊
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临床输血与检验

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (5): 495-498.DOI: 10.3969/j.issn.1671-2587.2016.05.030

• 技术与方法 • 上一篇    下一篇

几种血红蛋白测定方法的比较

聂晓绚, 林俊杰, 白虹, 孙蕴华, 徐蓓   

  1. 200051 上海市血液中心
  • 收稿日期:2016-06-17 出版日期:2016-10-20 发布日期:2017-05-19
  • 作者简介:聂晓绚（1981-）,女,上海人,主管技师,学士,主要从事实验室管理、后勤管理及人力资源管理工作,（Tel）021-62959500,13816376660（E-mail）niexiaoxuan@sbc.org.cn。

Comparisons of Several Methods for Hemoglobin Determination

NIE Xiao-xuan, LIN Jun-jie, BAI Hong, et al   

  1. Shanghai Blood Center,Shanghai 200051
  • Received:2016-06-17 Online:2016-10-20 Published:2017-05-19

PDF

2444

被引次数

9

摘要： 目的 选用较可靠的定量测试法测定献血者血红蛋白浓度,进一步提高献血者血红蛋白检测的精准率和可靠性。方法 以血红蛋白检测的金标准法氰化高铁（HiCN）法为基准,应用血细胞分析仪为对照组,对血红蛋白目测试剂（硫酸铜比重法）和干式血红蛋白检测试纸进行对比试验。结果 选择置信度P=95%对151例数据样本进行回归分析,优利特检测（y）与Sysmex基准（x）比较,y=0.892 2x+15.979,R2=0.7461;Hemocue检测（y）与Sysmex基准（x）比较,y=0.949 4x+15.018,R2=0.838 1。结论 采用干化学法检测血红蛋白,在灵敏度和稳定性方面均能与血细胞分析仪相媲美,相应的精密度、准确度及线性完全符合检测需求,特别针对血站的初筛检验具有实际意义。

关键词: 血红蛋白检测, 定量测定方法, 氰化高铁法

Abstract: Objective To choose a more reliable method to determine the hemoglobin concentration of blood donors in order to further improve the accuracy. Methods On the basis of gold standard method for the detection of hemoglobin (HiCN method), we used blood cell analyzer as the control group. Comparison test was carried out between visual reagent on hemoglobin (copper sulfate proportion) and dry hemoglobin test paper. Results Regression analysis of 151 cases of data samples with confidence degree P =95% was carried out. Comparison of Uritest detection(y) and Sysmex reference(x), y=0.892 2x+15.979, R2=0.7461; comparison of Hemocue detection(y) and Sysmex reference(x), y=0.949 4x+15.018, R2 =0.838 1. Conclusion Determination of hemoglobin with dry hemoglobin test paper is a method with similar sensitivity and stability with that of the method using blood cell analyzer. The precision, accuracy and linear degree fully meet the needs of testing. It has practical significance in especially blood screening test in blood banks.

Key words: Hemoglobin determination, Quantitative determination, HiCN method

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