关键词: 核酸检测, 乙肝/丙肝/艾滋病毒, 无偿献血

Abstract: Objective To analyze NAT used in the blood donation screening in Fuzhou areas,evaluate the necessity of NAT for blood donation testing and the difference of proficiency after reagent updating. Methods All of the specimens were tested by Procleix TIGRIS NAT System for HBV/HCV/HIV-1, and HBsAg, anti-HCV, anti-HIV/P24, anti-TP tested by two different companies' ELISA kits. Furthermore, the NAT reactive specimens were further confirmed by discriminatory NAT.Results 491 159 blood donors' specimens were tested from 2012 to 2017, the ELISA negative and NAT reactive specimens were 1 637, after discriminatory NAT 477 were HBV positive and 2 were HIV positive. Updating of the reagent since 2015, NAT reactive rates were improved from 0.28% to 0.41%, and the rates of discriminatory improved from 25.61% to 33.89%. Conclusion The window period of blood transfusion infection could be shorten and the blood safety improved after NAT used for blood screening. The sensitivity of the NAT reagent was improved after updating.

Key words: NAT, HBV/HCV/HIV-1, Blood donation