血小板裂解物体外抑菌活性及其与抗生素的协同效应研究*

doi:10.3969/j.issn.1671-2587.2024.04.003

临床输血与检验 ›› 2024, Vol. 26 ›› Issue (4): 446-450.DOI: 10.3969/j.issn.1671-2587.2024.04.003

血小板裂解物体外抑菌活性及其与抗生素的协同效应研究^*

冯慧慧, 林丹, 俞露, 邓刚

宁波市中心血站,浙江宁波 315010

收稿日期:2024-06-20 出版日期:2024-08-20 发布日期:2024-09-23
通讯作者: 邓刚,主要从事输血安全方面研究,（E-mail）dgflying@126.com。
作者简介:冯慧慧,主要从事血小板功能方面研究,（E-mail）249517853@qq.com。
基金资助:
*本课题受宁波市卫生健康科技计划项目（No.2023Y35）资助

Antibacterial Effect of Platelet Lysate (PL) in Vitro and Its Synergistic Effect with Antibiotics

FENG Huihui, LIN Dan, YU Lu, DENG Gang

Ningbo Central Blood Station, Ningbo 315010

Received:2024-06-20 Online:2024-08-20 Published:2024-09-23

摘要/Abstract

摘要： 目的通过体外实验探讨血小板裂解物（PL）的体外抑菌活性及其与抗生素的协同效应。方法选取大肠杆菌、肺炎克雷伯杆菌,采用微孔板法与牛津杯打孔法进行PL体外抑菌实验,微孔板法中设置庆大霉素(GM)、富血小板血浆（PRP）阳性对照,贫血小板血浆（PPP）阴性对照,测定OD值,计算抑菌率,牛津杯打孔法测定抑菌圈直径。结果对大肠杆菌的抑菌率GM>PL>PRP>PPP(P<0.05）,且随着PL浓度的降低,抑菌率逐渐降低;PL对大肠杆菌的抑菌率在4小时达到高峰,随着时间的延长,抑菌率逐渐降低。PL与庆大霉素的联用增强了庆大霉素的抗菌作用,PL+GM抑菌圈直径>GM抑菌圈直径。PL对肺炎克雷伯杆菌的抑菌作用GM+PL>GM>PL。结论 PL具有一定的抑菌作用,但弱于抗生素,其抑菌作用随着时间的延长及浓度的降低而逐渐减弱,并具有协同抗菌作用。

关键词: 富血小板血浆, 血小板裂解物, 抑菌实验, 抑菌率

Abstract: Objectives To investigate the antibacterial effects of platelet lysate (PL) in vitro and its synergistic effect with antibiotics. Methods Escherichia coli (E. coli) and K. pneumoniae were selected for PL in vitro antibacterial experiment by microplate method and Oxford cup drilling method. Gentamicin (GM), platelet rich plasma (PRP) positive control and platelet poor plasma (PPP) negative control was set in microplate method, OD value was determined, and antibacterial rate was calculated. The diameter of the antibacterial circle was determined by Oxford cup drilling method. Results The antibacterial rate against E. coli was found to be GM>PL>PRP>PPP (P<0.05), and the inhibition rate gradually decreased with the decrease of PL concentration; The antibacterial rate of PL on E. coli reached its peak at 4 hours, and gradually decreased with the prolongation of time. The combination of PL and GM enhanced the antibacterial effect of GM, with PL+GM zone of inhibition greater than GM zone of inhibition. The antibacterial effect against K. pneumoniae was found to be GM+PL>GM>PL. Conclusion PL has a certain antibacterial effect, but weaker than antibiotics. Its antibacterial effect gradually weakens with the prolongation of time and the decrease of concentration, and has a synergistic antibacterial effect.

Key words: Platelet-rich plasma, Platelet lysate, Antibacterial experiment, Antibacterial rate

中图分类号:

R457

冯慧慧, 林丹, 俞露, 邓刚. 血小板裂解物体外抑菌活性及其与抗生素的协同效应研究^*[J]. 临床输血与检验, 2024, 26(4): 446-450.

FENG Huihui, LIN Dan, YU Lu, DENG Gang. Antibacterial Effect of Platelet Lysate (PL) in Vitro and Its Synergistic Effect with Antibiotics[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(4): 446-450.

参考文献

[1] KIM D H,JE Y J,KIM C D,et al.Can platelet-rich plasma be used for skin rejuvenation? evaluation of effects of platelet-rich plasma on human dermal fibroblast[J]. Ann Dermatol,2011,23(4):424-431.
[2] VARSHNEY S,DWIVEDI A,PANDEY V.Antimicrobial effects of various platelet rich concentrates-vibes from in-vitro studies-a systematic review[J]. J Oral Biol Craniofac Res,2019,9(4):299-305.
[3] 郭彦杰,仇建军,张长青. 富血小板血浆治疗下肢慢性难愈合伤口47例随访研究[J]. 中国修复重建外科杂志, 2008,22(11):1301-1305.
[4] XIONG G,LINGAMPALLI N,KOLTSOV J C B,et al. Men and women differ in the biochemical composition of platelet-rich plasma[J]. 2018,46(2):409-419.
[5] 林燕,张宝献,刘春兰,等. 实验室几种常用菌悬液制备方法的探讨[J]. 广东化工,2021,48(21):65.
[6] 刘广亚,许育兵,张喻,等. 不同冻融条件下血小板裂解液的制备[J]. 中国输血杂志,2019,32(6):545-548.
[7] 宋明珠,宋金凤,郭刘艺,等. 富血小板血浆与抗生素联合应用在抑制大肠埃希菌中的作用[J]. 临床血液学杂志,2023,36(4):271-274.
[8] YEAMAN M R.Platelets in defense against bacterial pathogens[J]. Cell Mol Life Sci,2010,67(4):525-544.
[9] BADADE P S,MAHALE S A,PANJWANI A A,et al.Antimicrobial effect of platelet-rich plasma and platelet-rich fibrin[J]. Indian J Dent Res,2016,27(3):300-304.
[10] MARIANI E,FILARDO G,CANELLA V,et al.Platelet-rich plasma affects bacterial growth in vitro[J]. Cytotherapy,2014,16(9):1294-1304.
[11] MORRIS J,KELLY N,ELLIOTT L,et al.Evaluation of bacteriophage anti-biofilm activity for potential control of orthopedic implant-related infections caused by Staphylococcus aureus[J]. Surg Infect,2019,20(1):16-24.
[12] CETINKAYA R A,YILMAZ S,ÜNLÜ A,et al.The efficacy of platelet-rich plasma gel in MRSA-related surgical wound infection treatment:an experimental study in an animal model[J]. Eur J Trauma Emerg Surg,2018,44(6):859-867.
[13] D'ASTA F,HALSTEAD F,HARRISON P,et al. The contribution of leucocytes to the antimicrobial activity of platelet-rich plasma preparations:a systematic review[J]. Platelets,2018,29(1):9-20.
[14] ANITUA E,ALONSO R,GIRBAU C,et al.Antibacterial effect of plasma rich in growth factors (PRGF®-Endoret®) against Staphylococcus aureus and Staphylococcus epidermidis strains[J]. Clin Exp Dermatol,2012,37(6):652-657.
[15] MARIANI E,CANELLA V,BERLINGERI A,et al.Leukocyte presence does not increase microbicidal activity of Platelet-rich Plasma in vitro[J]. BMC Microbiol,2015,15(1):149.
[16] MAGHSOUDI O,RANJBAR R,MIRJALILI S H,et al.Inhibitory activities of platelet-rich and platelet-poor plasma on the growth of pathogenic bacteria[J]. Iran J Pathol,2017,12(1):79-87.
[17] ZHANG W,GUO Y,KUSS M,et al.Platelet-rich plasma for the treatment of tissue infection:preparation and clinical evaluation[J]. Tissue Eng Part B Rev,2019,25(3):225-236.
[18] 吕品,叶露露,单桂秋,等. 富血小板血浆对痤疮丙酸杆菌的体外抑菌实验研究[J]. 中国输血杂志,2016,29(6):558-560.

血小板裂解物体外抑菌活性及其与抗生素的协同效应研究^*

Antibacterial Effect of Platelet Lysate (PL) in Vitro and Its Synergistic Effect with Antibiotics

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

在线期刊

访问统计

联系我们

[1]	赵子悦, 黄韦华, 查占山. 富血小板血浆与间充质干细胞的联合应用在再生医学中的前景^*[J]. 临床输血与检验, 2024, 26(3): 420-426.
[2]	中国生物医学工程学会血液疗法与工程分会, 中国整形美容协会毛发医学分会, 富血小板血浆创新技术脱发治疗项目组. 自体单采富血小板血浆在脱发治疗中的临床应用专家共识^*[J]. 临床输血与检验, 2024, 26(1): 1-7.
[3]	陈旭升, 杨永涛. 富血小板血浆凝胶在5例糖尿病患者难愈性植入物外露创面的临床应用[J]. 临床输血与检验, 2024, 26(1): 123-126.
[4]	周振宇, 黄象艳. 附加侧板联合富血小板血浆治疗股骨骨折髓内固定后骨不连^*[J]. 临床输血与检验, 2023, 25(5): 648-653.
[5]	赵丽坤, 陈婷婷, 梁峰, 卓海龙, 骆群. 1例同种异体富血小板血浆在直肠吻合口瘘治疗中的应用[J]. 临床输血与检验, 2023, 25(5): 685-687.
[6]	肖军, 雷慧芬, 李翠莹. 血小板裂解液与血小板凝胶生长因子谱的表达差异研究^*[J]. 临床输血与检验, 2023, 25(4): 450-455.
[7]	王自林, 刘宏杰, 张娅, 祝丽丽. 水凝胶负载PRP治疗慢性难愈性创面的研究进展^*[J]. 临床输血与检验, 2023, 25(4): 564-571.
[8]	中国输血协会临床输血管理学专业委员会. 自体单采富血小板血浆治疗薄型子宫内膜的专家共识[J]. 临床输血与检验, 2023, 25(3): 289-296.
[9]	张强, 岳宪虎, 李瑞, 黄象艳. 富血小板血浆在髌骨外侧高压症治疗中的应用^*[J]. 临床输血与检验, 2023, 25(3): 348-353.
[10]	刘琴, 沈先涛, 徐春芬, 王阳, 杨柳春, 胡红兵. 自体富血小板血浆治疗儿童病理性骨折疗效观察^*[J]. 临床输血与检验, 2023, 25(3): 354-357.
[11]	巩蜜蜜, 汪海宁, 吴磊, 邵小宝, 周琳, 杨圆, 王华, 高凤威, 戴宇东, 朱培元. 同种异体富血小板血浆用于慢性难愈性创面的临床研究^*[J]. 临床输血与检验, 2023, 25(3): 368-374.
[12]	毛平平, 王淑君, 罗开云, 齐清, 赵广超, 栾建凤. 冰冻PRP和冻干PRP的抗菌作用研究^*[J]. 临床输血与检验, 2022, 24(6): 705-709.
[13]	魏燕, 刘宽, 李祥军, 彭娟, 王瑞含, 刘久波. PRP治疗踝关节创伤性滑膜炎疗效观察^*[J]. 临床输血与检验, 2022, 24(6): 725-729.
[14]	罗开云, 王淑君, 毛平平, 孙杨子, 齐清, 栾建凤. 不同保存方式对PRP相关生长因子影响的研究*[J]. 临床输血与检验, 2022, 24(1): 70-73.
[15]	杨菁, 周谋, 林放, 施琳颖, 李艳辉, 李文丹, 单桂秋. 负载PRP壳聚糖-明胶复合敷料止血和促愈合效果的初步评价^*[J]. 临床输血与检验, 2021, 23(5): 612-616.